However, a new era has emerged with the advent of long-read sequencing (LRS), which offers substantial improvements over its predecessor, short-read sequencing (SRS). LRS produces reads that are several kilobase
Shotgun DNA sequencing has been used to build contiguous sequence information of entire chromosomes1. However, previous sequencing reads were too short to resolve the order of repetitive sequences, because the repeats were longer than an individual read, or sequence variations between two tandem copie...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
Transcriptome sequencing revolutionized the analysis of gene expression, providing an unbiased approach to gene detection and quantification that enabled the discovery of novel isoforms, alternative splicing events and fusion transcripts. However, although short-read sequencing technologies have surpassed the li...
Through four decades’ development, DNA sequencing has inched into the era of single-molecule sequencing (SMS), or the third-generation sequencing (TG
Advances in clinical genetic testing, including the introduction of exome sequencing, have uncovered the molecular etiology for many rare and previously unsolved genetic disorders, yet more than half of individuals with a suspected genetic disorder remai
Long-read DNA sequencing captured entire integrated HBV sequences including multiple kilobases of flanking host sequence within single reads. This resolution allowed us to describe integration events flanked by 2 different host chromosomes, indicating that integrated HBV DNA are associated with inter-...
Keywords:hybrid assembly, bacterial genomics, long-read sequencing,Enterobacteriaceae, plasmid assembly 摘要 Illumina测序允许快速、廉价和准确的全基因组细菌分析,但是短读(300 bp)通常不能实现完整的基因组组装。 长读测序大大有助于解决复杂的细菌基因组,特别是当结合短读Illumina数据(杂交组装)。
This article is a preprint and has not been certified by peer review [what does this mean?]. ABSTRACT BackgroundThe newest generation of DNA sequencing technology is highlighted by the ability to sequence reads hundreds of kilobases in length, and the increased availability of long read data has...
1,2 Short-read NGS does not support direct variant phasing, leaving analysis highly dependent on reference genomes, which are known to be imperfect and problematic for genes with a high degree of heterogeneity among different populations.1, 2, 3 Long-read sequencing (LRS) platforms may be able...