However, a new era has emerged with the advent of long-read sequencing (LRS), which offers substantial improvements over its predecessor, short-read sequencing (SRS). LRS produces reads that are several kilobases long, enabling researchers to obtain more complete and contiguous gen...
Shotgun DNA sequencing has been used to build contiguous sequence information of entire chromosomes1. However, previous sequencing reads were too short to resolve the order of repetitive sequences, because the repeats were longer than an individual read, or sequence variations between two tandem copie...
PacBio long-read sequencing enables the generation of lengthy reads that encompass one or multiple HPV DNA fragments flanked by the human genome on both ends. This characteristic enhances the reliability and facilitates direct mapping of the reads across HPV breakpoints, thereby facilitating the elucida...
a protein25, causes nonsense-mediated decay (NMD)88, contains repetitive elements93, is a transmembrane protein94, contains a domain motif95, contains a signal peptide96, or has intrinsically disordered regions (IDRs)97 in silico based on the nucleotide sequence obtained by long-read sequencing. ...
Through four decades’ development, DNA sequencing has inched into the era of single-molecule sequencing (SMS), or the third-generation sequencing (TG
Advances in clinical genetic testing, including the introduction of exome sequencing, have uncovered the molecular etiology for many rare and previously unsolved genetic disorders, yet more than half of individuals with a suspected genetic disorder remai
Keywords:hybrid assembly, bacterial genomics, long-read sequencing,Enterobacteriaceae, plasmid assembly 摘要 Illumina测序允许快速、廉价和准确的全基因组细菌分析,但是短读(300 bp)通常不能实现完整的基因组组装。 长读测序大大有助于解决复杂的细菌基因组,特别是当结合短读Illumina数据(杂交组装)。
et al. Long-Read Sequencing Analysis Revealed the Impact of Forest Conversion on Soil Fungal Diversity in Limu Mountain, Hainan. Microb Ecol 86, 872–886 (2023). https://doi.org/10.1007/s00248-022-02129-y Download citation Received17 February 2022 Accepted23 October 2022 Published04 November...
This article is a preprint and has not been certified by peer review [what does this mean?]. Abstract Full Text Info/History Metrics Preview PDF Abstract SummaryLong read sequencing platforms, which include the widely used Pacific Biosciences (PacBio) platform and the emerging Oxford Nanopore plat...
Long-read DNA sequencing captured entire integrated HBV sequences including multiple kilobases of flanking host sequence within single reads. This resolution allowed us to describe integration events flanked by 2 different host chromosomes, indicating that integrated HBV DNA are associated with inter-...