All short-read sequencing technologies have a common limitation – the inability to sequence long stretches of DNA. To sequence a large stretch of DNA using NGS, such as a human genome, the strands have to be fragmented and amplified.Bioinformatic programsare then used to assemble these random ...
Long-read and short-read sequencing technologies offer competing advantages for eukaryotic genome sequencing projects. Combinations of both may be appropriate for surveys of within-species genomic variation. Methods We developed a hybrid assembly pipeline called “Alpaca” that can operate on 20X long-re...
Whole genome/transcriptome sequencing (WGS/WTS) provides comprehensive insights, while targeted sequencing is more cost-effective and sensitive. In comparison to short-read sequencing, which still dominates the field due to high speed and cost-effectiveness, long-read sequencing can overcome alignment ...
BMI and DSE reproductive glands. As the dataset contains both long-read and short-read sequencing data, this rich sequencing dataset paves the avenue for quantifying gene or transcript expression, evaluating alternative splicing, delving novel transcripts, and improving the annotation of the pig ...
Antimicrobial resistance (AMR) is a major public health threat. Plasmids are able to transfer AMR genes among bacterial isolates. Whole genome sequencing (WGS) is a powerful tool to monitor AMR determinants. However, plasmids are difficult to reconstruct
Sentieon | 每周文献-Long Read Sequencing(长读长测序)-第七期,研究者通过将PacBio长读长数据进行组装后对基因组进行repeat和gene注释。然后,利用测序或者从公开发表文献中下载的棉花基因组数据进行比对、变异检测、联合基因分型对亚洲栽培棉和非洲亚种进行比较。此过
在2016年,首届瓶中基因组(GIAB)-precisionFDA真相挑战赛正式启幕,挑战参赛者从两个GIAB样本的短读长数据中识别出小变体。虽然HG001(也被称为NA12878)的基准测试数据已提前公布,但当时并未公开HG002的基准测试数据,这使得该挑战成为首个盲法种系变异检测挑战。其结果公开后,已被用作评估新变异检测方法的基准。尽管目...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...
Introduction: Genome-based analysis is crucial in monitoring antibioticresistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing is typically used to obtain incomplete draft genomes, while longread sequencing can obtain genomes of multidrug resistance (MDR) plasmids and track ...
Carex L., a grass genus commonly known as sedges, is distributed worldwide and contributes constructively to turf management, forage production, and ecological conservation. The development of next-generation sequencing (NGS) technologies has considerabl