Sequencing Data Analysis Biological Data Interpretation All Informatics Education Next-generation sequencing for beginners Curious about using NGS in your research? Learn the basics of next-generation sequencing and find tips for getting started. Learn about NGS Company About Us News & Events ...
the budding yeastSaccharomyces cerevisiaehas many isolates currently being sequenced with long reads. However, analyzing long-read sequencing data to produce high-quality genome assembly
目前这里有大量不同的DNA和RNA修饰,好多都是没有被检测到和研究的,长读长测序为研究这些修饰的分布和功能打开了新的大门。 Lucas, M.C., Novoa, E.M. Long-read sequencing in the era of epigenomics and epitranscriptomics.Nat Methods20, 25–29 (2023).https://doi.org/10.1038/s41592-022-01724-8 ...
Several studies have demonstrated the regulatory roles of read-through transcripts in tumorigenesis and development. However, limited by the read length of next-generation sequencing, discovery of read-through transcripts has been slow. For long but also erroneous third-ge...
在2016年,首届瓶中基因组(GIAB)-precisionFDA真相挑战赛正式启幕,挑战参赛者从两个GIAB样本的短读长数据中识别出小变体。虽然HG001(也被称为NA12878)的基准测试数据已提前公布,但当时并未公开HG002的基准测试数据,这使得该挑战成为首个盲法种系变异检测挑战。其结果公开后,已被用作评估新变异检测方法的基准。尽管目...
Sentieon | 每周文献-Long Read Sequencing(长读长测序)-第七期,研究者通过将PacBio长读长数据进行组装后对基因组进行repeat和gene注释。然后,利用测序或者从公开发表文献中下载的棉花基因组数据进行比对、变异检测、联合基因分型对亚洲栽培棉和非洲亚种进行比较。此过
2023年1月12号,NatureMethod报道2022年最值得关注的方法——长读段测序(Long-read sequencing)。 今年6月,Nature Method上发表关于一篇T2T的文章,并强调端粒到端粒(T2T)联盟成功地提出了第一个完整的人类基因组。这一成就是通过广泛的实验和计算努力取得的。其中,长读测序是负责生成T2T数据的主要测序技术,可以说为...
NanoPack: visualizing and processing long-read sequencing data. Bioinformatics 34, 2666–2669 (2018). Article PubMed PubMed Central CAS Google Scholar Alasoo, K. Wiggleplotr: Make read coverage plots from bigwig files. Bioconductor https://doi.org/10.18129/B9.bioc.wiggleplotr (2017). Pert...
ARCS and ARKS are genome sequence assembly scaffolders using linked and long read sequencing data Run modes - cheat sheet ARCS can be run in 4 modes: ARCS(default) uses alignments of linked reads to the input contigs ARCS-long (arcs-long) uses alignments of long reads to the input contigs...
As long-read sequencing technologies are becoming increasingly popular, a number of methods have been developed for the discovery and analysis of structural variants (SVs) from long reads. Long reads enable detection of SVs that could not be previously d