Long-Read Sequencing Microarray Technology Sequencing Method Explorer All Technologies Next-generation sequencing for beginners Curious about using NGS in your research? Learn the basics of next-generation sequencing and find tips for getting started. Learn about NGS Illumina Research & Innovation Geno...
Long-read sequencing technologies can enable the resolution of structurally complex or repetitive genome regions that are difficult or impossible to resolve with short-read sequencing. Pairing the power of long-read sequencing platforms with targeted hybridization capture technology allows researchers to effi...
Long-read sequencingPacBioOxford Nanopore TechnologyShort-read sequencingInfectious diseaseCancer genomicsStructural variantsThe extensive applications of next-generation sequencing (NGS) technologies have transformed the field of genetics and genomics research over time. In recent years, different long-read ...
目前这里有大量不同的DNA和RNA修饰,好多都是没有被检测到和研究的,长读长测序为研究这些修饰的分布和功能打开了新的大门。 Lucas, M.C., Novoa, E.M. Long-read sequencing in the era of epigenomics and epitranscriptomics.Nat Methods20, 25–29 (2023).https://doi.org/10.1038/s41592-022-01724-8 ...
Nature Methods 认为，随着技术不断创新进步的步伐，长读长测序实现更长、更准并且更经济高效将是未来值得期待的重点！未来，齐碳科技将不断自主创新，继续向纳米孔基因测序科研无人区挺进，持续提升国产测序仪性能，探索纳米孔测序技术的更多可能性，并加快推动这一高精尖技术真正走入寻常百姓家，为人类健康贡献“中国...
Sentieon | 每周文献-Long Read Sequencing(长读长测序)-第七期,研究者通过将PacBio长读长数据进行组装后对基因组进行repeat和gene注释。然后,利用测序或者从公开发表文献中下载的棉花基因组数据进行比对、变异检测、联合基因分型对亚洲栽培棉和非洲亚种进行比较。此过
在2016年,首届瓶中基因组(GIAB)-precisionFDA真相挑战赛正式启幕,挑战参赛者从两个GIAB样本的短读长数据中识别出小变体。虽然HG001(也被称为NA12878)的基准测试数据已提前公布,但当时并未公开HG002的基准测试数据,这使得该挑战成为首个盲法种系变异检测挑战。其结果公开后,已被用作评估新变异检测方法的基准。尽管目...
The PacBio and ONT sequencing are able to read full-length transcripts and allow the direct study of base modifications on both DNA and RNA molecules. Nanopore technology is able to sequence RNA directly. LRS has revealed a much more complex viral transcriptome. Among other capabilities, these ...
BackgroundThe newest generation of DNA sequencing technology is highlighted by the ability to sequence reads hundreds of kilobases in length, and the increased availability of long read data has democratized the genome sequencing and assembly process. PacBio and Oxford Nanopore Technologies (ONT) have...
2023年1月12号,NatureMethod报道2022年最值得关注的方法——长读段测序(Long-read sequencing)。 今年6月,Nature Method上发表关于一篇T2T的文章,并强调端粒到端粒(T2T)联盟成功地提出了第一个完整的人类基因组。这一成就是通过广泛的实验和计算努力取得的。其中,长读测序是负责生成T2T数据的主要测序技术,可以说为...