Here, we present a modular computational framework named long-read sequencing data analysis for yeasts (LRSDAY), the first one-stop solution that streamlines this process. Starting from the raw sequencing reads, LRSDAY can produce chromosome-level genome assembly and comprehensive genome annotation ...
the budding yeastSaccharomyces cerevisiaehas many isolates currently being sequenced with long reads. However, analyzing long-read sequencing data to produce high-quality genome assembly
In this study, we compared the genotyping of mitochondrial DNA variants in three samples using PacBio's Sequel (Pacific Biosciences Inc., Menlo Park, CA, USA) long-read sequencing and illumina's HiSeqX10 (illumine Inc., San Diego, CA, USA) short-read sequencing data. We concluded that, ...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...
28Branches19Tags Code README GPL-3.0 license ARCS Scaffolding genome sequence assemblies using linked or long read sequencing data Contents Description ARCS and ARKS are genome sequence assembly scaffolders using linked and long read sequencing data ...
The debate between short-read and long-read sequencing is an ongoing one, but it is clear that both technologies have their own unique benefits. For the most comprehensive results, it is best to combine the two together. By doing so, you can get the most complete picture of your data whi...
This script performs data extraction from Oxford Nanopore sequencing data in the following formats: fastq files (can be bgzip, bzip2 or gzip compressed) fastq files generated by albacore, guppy or MinKNOW containing additional information (can be bgzip, bzip2 or gzip compressed) ...
COVID-19 sequencing Application Dataset Data type PacBio system SARS-CoV-2 surveillance Omicron Samples HiFi reads Sequel II system SARS-CoV-2 surveillance Synthetic RNA controls HiFi reads Sequel II system SARS-CoV-2 surveillance Surveillance samples HiFi reads Sequel II system ...
(Fig.1b). This identified trends in the evolution of research interests: likely due to the modest initial throughput of long-read sequencing technologies, the majority of tools were tested on non-human data; tools for de novo assembly, error correction, and polishing categories have received ...
Here we describe NanoPack, a set of tools developed for visualization and processing of long read sequencing data from Oxford Nanopore Technologies and Pacific Biosciences. Availability and Implementation: The NanoPack tools are written in Python3 and released under the GNU GPL3.0 Licence. The source...