Long-read sequencing technologies promise to improve current annotations, paving the way towards a complete annotation of lncRNAs expressed throughout a human lifetime. This is a preview of subscription content, access via your institution Access options Access Nature and 54 other Nature Portfolio ...
During the last years, technological developments in DNA sequencing read length have lead not only to an improvement in the quality and completeness of reference genomes15,16,17,18,19,20, but also to a significant rise in the number of high-quality genomes for multiple individuals of the same...
The present study provides a comprehensive resource of five BMI and DSE reproductive glands. As the dataset contains both long-read and short-read sequencing data, this rich sequencing dataset paves the avenue for quantifying gene or transcript expression, evaluating alternative splicing, delving novel...
2]. While short-read sequencers such as Illumina’s NovaSeq, HiSeq, NextSeq, and MiSeq instruments [3–5]; BGI’s MGISEQ and BGISEQ models [6]; or Thermo Fisher’s Ion Torrent sequencers [7,8] produce reads of up to 600 bases, long-read sequencing technologies...
To better understand the full-length transcriptome of the nucleus accumbens (NAc)—a key brain reward region—in chronic cocaine treatment, we perform the first single molecule, long-read sequencing analysis using the Iso-seq method to detect 42,114 unique transcripts from mouse NAc polyadenylated...
Long-read transcriptome sequencing allows the discovery of fusions with their full-length isoform structure. However, due to higher sequencing error rates, fusion finding algorithms designed for short reads do not work. Here we present JAFFAL, to identify fusions from long-read transcriptome sequencing...
We provided the SV callsets of the HG002 human sample produced by cuteSV form three different long-read sequencing platforms (i.e. PacBio CLR, PacBio CCS, and ONT PromethION). You can download them at: Please cite the manuscript of cuteSV before using these callsets. ...
The human transcriptome annotation is regarded as one of the most complete of any eukaryotic species. However, limitations in sequencing technologies have biased the annotation toward multi-exonic protein coding genes. Accurate high-throughput long read
Long-read sequencing technologies have substantially improved the assemblies of many isolate bacterial genomes as compared to fragmented short-read assemblies. However, assembling complex metagenomic datasets remains difficult even for state-of-the-art long-read assemblers. Here we present metaFlye, which...
Transcriptomic long-read sequencing presents unique opportunities to overcome the shortcomings of short-read technologies for gene fusion detection while introducing new challenges. Results We present Genion, a sensitive and fast gene fusion detection method that can also detect read-through events. We ...