The present study provides a comprehensive resource of five BMI and DSE reproductive glands. As the dataset contains both long-read and short-read sequencing data, this rich sequencing dataset paves the avenue for quantifying gene or transcript expression, evaluating alternative splicing, delving novel...
compared to references42, e.g. the one now provided for GMB. subtilis2014-3557. If this comparison shows many unnatural associations and/or AMR genes that have not been found before in related GMMs or wild-types, then it is advisable to perform long-read sequencing to fully characterize the...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...
Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences. Here we demonstrate a method to reconstruct the sequences of individual nucle...
These limitations may now be addressed by long-read sequencing technologies such as Nanopore, either in combination with another technology as in this study or as a first line approach. These have the advantage of reads of 10–100 Kb allowing for more accurate mapping particularly over repetitive...
Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding We describe the genome sequencing of an anonymous individual of African origin using a novel ligation-based sequencing assay that enables a unique form of ... ...
Added option (--merge_paired) to accommodate cases where users have multiple read sets for the same sample. If this flag is used, SRST2 will assume that all the input reads belong to the same sample, and outputs will be named as[prefix]__combined.xxx, where SRST2 was run using--outpu...
Molecular cloning and sequencing of two ‘short chain’ and two ‘long chain’ K+channel-blocking peptides from the Chinese scorpionButhus martensiiKarsch (1999) Molecular cloning and sequencing of two 'short chain' and two 'long chain' K(+) channel-blocking peptides from the Chinese scorpion...
PolyAtailor is a user-friendly R package for measuring poly(A) tail length from NGS-based short-read and PacBio-based long-read sequencing data. Core functions of Tail_map and Tail_scan provided in PolyAtailor can profile tails with or without using a re
Recent advances in throughput and accuracy mean that the Oxford Nanopore Technologies PromethION platform is a now a viable solution for genome sequencing. Much of the validation of bioinformatic tools for this long-read data has focussed on calling germ