Whole genome/transcriptome sequencing (WGS/WTS) provides comprehensive insights, while targeted sequencing is more cost-effective and sensitive. In comparison to short-read sequencing, which still dominates the field due to high speed and cost-effectiveness, long-read sequencing can overcome alignment ...
RNA sequencing (RNA-seq) that utilises short-read next-generation sequencing (NGS) or long-read sequencing (such as PacBio and Oxford Nanopore Technologies sequencing) is a powerful tool in understanding transcriptional diversity and regulation during various biological processes, including hematopoiesis2...
The present study provides a comprehensive resource of five BMI and DSE reproductive glands. As the dataset contains both long-read and short-read sequencing data, this rich sequencing dataset paves the avenue for quantifying gene or transcript expression, evaluating alternative splicing, delving novel...
However, it has limitations in accurately assembling large genomes and identifying complex genomic variations, such as structural variations and repetitive sequences, due to the short read length. To overcome these limitations, long-read sequencing technologies, such as Pacific Biosciences (PacBio) and...
Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences. Here we demonstrate a method to reconstruct the sequences of individual nucle...
(SBB) chemistry delivers a breakthrough in sequencing accuracy. Compared to sequencing by synthesis (SBS) utilized by traditional short-read sequencers, SBB chemistry gives ~15x lower error rates at any given cycle so that you can achieve near-perfect accuracy in short-read sequencing without ...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...
Compared to sequencing by synthesis (SBS) utilized by traditional short-read sequencers, SBB chemistry gives ~15x lower error rates at any given cycle so that you can achieve near-perfect accuracy in short-read sequencing without having to sequence at unnecessary depths....
With the rapid expansion of high-throughput sequencing technologies including long-read WGS, genome-wide characterisation of SVs with high precision has been achieved [1], facilitating the study of more complex forms of SVs. Therefore, in the present study, we sought to investigate the role of ...
Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding We describe the genome sequencing of an anonymous individual of African origin using a novel ligation-based sequencing assay that enables a unique form of ... ...