compared to references42, e.g. the one now provided for GMB. subtilis2014-3557. If this comparison shows many unnatural associations and/or AMR genes that have not been found before in related GMMs or wild-types, then it is advisable to perform long-read sequencing to fully characterize the...
Resolving these allelic differences by chromosomal copy, also known as phasing, is achievable on a short-read sequencer when using a library preparation method that captures long-range genomic information. TELL-Seq is a library preparation that captures long-range genomic information with the aid of...
This revolutionary sequencer allows researchers to see the things that can be seen with a conventional short-read exome or whole genome (SNPs, etc.) –and takes it even further by enabling access to more complete, 99.9% accurate sequencing coverage (including genomic dark regions) with superior...
WGS data with PCR (WGS_PCR_1 and WGS_PCR_2) were generated by the Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Sydney, Australia, and sequenced on a HiSeq X Ten sequencer. The WES data (WES_PCR) were generated by the Australian Genome Research Facility, ...
with a goal of trying to better understand these understudied taxa. We performed long-read sequencing on three samples from participants in Bushbuckridge with an Oxford Nanopore MinION sequencer (Supplementary Table5, taxonomic composition of the three samples shown in Supplementary Fig.16). Samples ...
Sequencing: An Illumina HiSeq 2500 or HiSeq X Ten sequencer was used to sequence the small RNA libraries. The sequencing data were deposited in the Gene Expression Omnibus of the National Center for Biotechnology Information (NCBI) under accession number GSE95218. The oligo sequences of 3′ ad...
Single molecule sequencing (SMS) platforms enable base sequences to be read directly from individual strands of DNA in real-time. Though capable of long read lengths, SMS platforms currently suffer from low throughput compared to competing short-read sequencing technologies. Here, we present a novel...
Quantified libraries were multiplexed and loaded on an Illumina GAIIx sequencer at 5 pM. The Illumina libraries were run alongside a PhiX control lane on a two-lane partial flowcell run (42) with 25 nt read on the first read, 6 nt read in the barcode and 45 nt read on the reverse ...
Telomeres are environment-sensitive regulators of health and aging. Here,we present telomere DNA length analysis of two reef-building coral genera revealing that the long- and short-term water thermal regime is a key driver of between-colony variation ac
However, we also note that Mps1i pulse in the 1st mitosis could affect chromosome stability in a long-term and aneuploidy-independent manner, through not-yet-identified mechanisms unrelated to chromosome mis-segregation. Fig. 1: Aneuploid cells accumulate increasing genome instability and display ...