Short-read sequencing, also known as next-generation sequencing (NGS), is a high-throughput sequencing method that enables rapid and cost-effective analysis of DNA or RNA sequences. It involves breaking the DNA or RNA into short fragments and then simultaneously sequencing millions of these fragmen...
All short-read sequencing technologies have a common limitation – the inability to sequence long stretches of DNA. To sequence a large stretch of DNA using NGS, such as a human genome, the strands have to be fragmented and amplified.Bioinformatic programsare then used to assemble these random ...
Genome sequencing is an important tool for detecting disease-causing variants and several computational tools have been developed to analyze tandem repeats from genomic data, enabling the genotyping and the identification of expanded alleles. However, guidelines for conducting the analysis of the...
The accuracy of SBB’s novel sequencing approach has the potential to advance cancer diagnostics, drive higher fidelity for single-cell applications, and broadly enable clinical sequencing
Short Read Sequencing Analysis Workshop Day 3 – Using Compute Resources at BioFrontiers Institute In-class Slides – Jonathan DeMasi Outline For Today Questions about videos??? Brief review of compute cluster Brief review of job scripts and SBATCH headers Modules (Lmod) Transferring or Downloading...
The accuracy of SBB’s novel sequencing approach has the potential to advance cancer diagnostics, drive higher fidelity for single-cell applications, and broadly enable clinical sequencing
The direct detection of haplotypes from short-read DNA sequencing data requires changes to existing small-variant detection methods. Here, we develop a Bayesian statistical framework which is capable of modeling multiallelic loci in sets of individuals with non-uniform copy number. We then describe ...
Short-read sequencing tools. Contribute to imgag/ngs-bits development by creating an account on GitHub.
Haznedaroglu BZ, Reeves D, Rismani-Yazdi H, Peccia J: Optimization of de novo transcriptome assembly from high-throughput short read sequencing data improves functional annotation for non-model organisms. BMC Bioinformatics. 2012, 13: 170-10.1186/1471-2105-13-170. Central...
Measuring differential gene expression by short read sequencing: quantitative comparison to 2-channel gene expression microarrays. BMC Genomics. 2009; 10 :221.Bloom JS, Khan Z, Kruglyak L, Singh M, Caudy AA: Measuring differential gene expression by short read sequencing: quantitative comparison to ...