However, it has limitations in accurately assembling large genomes and identifying complex genomic variations, such as structural variations and repetitive sequences, due to the short read length. To overcome these limitations, long-read sequencing technologies, such as Pacific Biosciences (PacBio) and...
DETERMINATION OF STR LENGTH BY SHORT READ SEQUENCINGDescribed herein are methods, software, and systems of determining a repeat length of a short tandem repeat (STR) sequence at a given STR locus from nucleic acid sequence reads generated by short read technology.TANG, HAIBAO...
Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences. Here we demonstrate a method to reconstruct the sequences of individual nucle...
Sequencing by binding on the Onso system The Onso system is an integrated hardware and software short-read sequencing platform focused on delivering best-in-class accuracy, resulting in over 90 issued patents and numerous pending applications in the US and abroad. The Onso system utilizes SBB ...
we integrated single-molecule long-read sequencing with short-read sequencing to characterize the RNA landscape from five glands of Banna mini-pig inbred line (BMI) and Diannan small-ear pigs (DSE). We identified 110,996 full-length isoforms from 22,298 genes, and classified the alternative sp...
Cost-effective short-read sequencing technologies have been widely applied to generate high-quality microbial reference genomes from large cohorts of human gut microbiomes2,3,4. However, the short-read length (100–300bps) may not allow us to resolve intra-species repetitive regions and inter-...
Here, we combine the higher taxonomic resolution of near-full-length 16S rRNA gene amplicons with the economics and sensitivity of short-read sequencing to assay the abundance and identity of organisms that represent as little as 0.01% of sediment bacterial communities. We used a new version of ...
PenguiN a software to assemble short read sequencing data on a nucleotide level. In a first step it assembles coding sequences using the information from the translated protein sequences. In a second step it links them across non-coding regions. The main purpose of PenguiN is the assembly of...
which is not as confident as when the whole length of a read maps within the gene (reported if this value is below the cutoff specified (default--min_edge_depth 2), low values can be interpreted as indicating uncertainty in the result as we can’t confidently distinguish alleles that diffe...
(Fig.6a). We visualized reads of full-length transcripts both in nanopore and PacBio sequencing and found that all annotated isoforms were identified (Fig.6c). A 5′ alternative start site was found in the first exon of c-Myc. We used the short-read sequencing data to quantify the ...