Whole genome/transcriptome sequencing (WGS/WTS) provides comprehensive insights, while targeted sequencing is more cost-effective and sensitive. In comparison to short-read sequencing, which still dominates the field due to high speed and cost-effectiveness, long-read sequencing can overcome alignment ...
Short-read sequencing, also known as next-generation sequencing (NGS), is a high-throughput sequencing method that enables rapid and cost-effective analysis of DNA or RNA sequences. It involves breaking the DNA or RNA into short fragments and then simultaneously sequencing millions of these fragmen...
The present study provides a comprehensive resource of five BMI and DSE reproductive glands. As the dataset contains both long-read and short-read sequencing data, this rich sequencing dataset paves the avenue for quantifying gene or transcript expression, evaluating alternative splicing, delving novel...
RNA sequencing (RNA-seq) that utilises short-read next-generation sequencing (NGS) or long-read sequencing (such as PacBio and Oxford Nanopore Technologies sequencing) is a powerful tool in understanding transcriptional diversity and regulation during various biological processes, including hematopoiesis2...
The accuracy of SBB’s novel sequencing approach has the potential to advance cancer diagnostics, drive higher fidelity for single-cell applications, and broadly enable clinical sequencing
Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences. Here we demonstrate a method to reconstruct the sequences of individual nucle...
The accuracy of SBB’s novel sequencing approach has the potential to advance cancer diagnostics, drive higher fidelity for single-cell applications, and broadly enable clinical sequencing
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyi&#
Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding We describe the genome sequencing of an anonymous individual of African origin using a novel ligation-based sequencing assay that enables a unique form of ... ...
TRiCoLOR: tandem repeat profiling using whole-genome long-read sequencing data. Gigascience 9, giaa101 (2020). Article PubMed PubMed Central Google Scholar Guo, R. et al. RepLong: de novo repeat identification using long read sequencing data. Bioinformatics 34, 1099–1107 (2018). Article ...