Single-nuclei isolationsnRNA-seqArabidopsis thaliana seedlingsflower developmentantherSingle-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nuclei isolation combined with nanowell-based library ...
Single-cell RNA-seq is a powerful tool in decoding the heterogeneity in complex tissues by generating transcriptomic profiles of the individual cell. Here, we report a single-nuclei RNA-seq (snRNA-seq) transcriptomic study on human retinal tissue, which is composed of multiple cell types with di...
采用 DropSeq,DroNc-seq和10xChromium平台(平台差异详见Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems)分别进行单细胞和单细胞核转录组分析,比较不同的样品类型对单细胞结果产生的影响。
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
Single-nuclei RNA-seq (snuclRNA-seq) is an alternative to this methodology. Studies of mouse neural progenitor cells show that only small differences are present between total cellular and nuclear RNA profiles [9]. We sought to investigate the feasibility of generating and employing unsorted sn...
Single-nuclei RNA Profiling Reveals Disruption of Adipokine and Inflammatory Signaling in Adipose Tissue of Burn Patients Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zach...
UsingsinglenucleiforRNA-seqtocapturethetranscriptomeof postmortemneurons SugunaRaniKrishnaswami 1,9 ,RashelVGrindberg 2,9 ,MarkNovotny 1 ,PratapVenepally 3 , BenjaminLacar 4 ,KunalBhutani 1 ,SaraBLinker 4 ,SonPham 4 ,JenniferAErwin 4 ,JeremyA Miller 5 ,RebeccaHodge 5 ,JamesKMcCarthy 1 ,Marti...
vignettes DESCRIPTION LICENSE NAMESPACE README.md DeTREMis a modification of the MuSiC deconvolution algorithm optimized for single-nuclei reference data. How to citeDeTREM Please cite the following publication: Bulk brain tissue cell-type deconvolution with bias correction for single-nuclei RNA sequencin...
In comparison with single-cell RNA sequencing (scRNA-seq) library generated with live cells from freshly isolated human islets, the snRNA-seq library displayed comparable endocrine cellular composition and cell type signature gene expression. However, between these two types of libraries, differential ...