组会上同学分享了一篇实验生物学文章,有趣的是里面用到了RNA-seq,而且数据量不行,不过分析的点非常少,由此可以说明RNA-seq技术的常规化了。 背景介绍 多发性骨髓瘤(Multiple Myeloma,MM) 骨髓瘤又称浆细胞瘤,有多种类型,其中多发性骨髓瘤(Multiple Myeloma,MM)最为常见。 多发性骨髓瘤(MM)是B细胞恶性肿瘤,它...
RNA-Seq Based Transcriptome Analysis of Endothelial Differentiation of Bone Marrow Mesenchymal Stem CellsGene expressionEndothelial differentiationStem cellsEndothelial cellsObjective: The aim was to identify the change in gene expression between mesenchymal stem cells (MSCs) and induced endothelial cells (ECs...
9月29日,在国际顶级学术期刊《Nature》发表的一项研究中,来自英国威康信托基金会桑格研究所、香港中文大学、上海农科院、上海交通大学、剑桥大学和墨尔本大学等处的研究人员,通过对骨髓(BM)先天淋巴细胞(ILCs)进行单细胞RNA测序(scRNAseq),确定了不同的ILC前体细胞亚群,描述了不同的ILC发育阶段和途径,并发现PD-1可以...
Machine-learning and scRNA-Seq-based diagnostic and prognostic models illustrating survival and therapy response of lung adenocarcinoma Qingyu Cheng Weidong Zhao Tengchuan Jin Genes & Immunity(2024) The bone ecosystem facilitates multiple myeloma relapse and the evolution of heterogeneous drug resistant dis...
condition=factor(c(rep(“lung”,5),rep(“bone”,8),rep(“bone”,8),rep(“kidney”,4))) condition design<-model.matrix(~-1+condition) design colnames(design)<-c("liver","lung","bone","kidney") contrast.matrix<-makeContrasts(liver-kidney,lung-kidney,bone-kidney,levels = design) ...
单细胞测序分析提供生物组织的高分辨率测量。因此,此类技术可以帮助破译和理解细胞异质性和生物过程的动态。相应的研究包括量化细胞命运以及识别驱动该过程的基因。然而,由于在经典单细胞 RNA 测序(scRNA-seq)方案中进行测序时细胞会被破坏,因此无法跟踪它们的发育情况,例如随时间变化的基因表达谱。因此,需要根据测量的快照...
In this study, we used RNA-seq to characterize the genome-wide expression portrait of human MSCs derived from bone marrow (BMs), adipose tissue (AMs), and palatine tonsil (TMs). Further, we determined the phenotypes of BMs, AMs, and TMs to verify common MSC surface markers as well as ...
之前已经详细讲解了通过该工具对GEO数据进行差异基因筛选(酸菜:找不到差异基因?这个憨憨版在线分析工具了解一下),本文则会详细介绍NetworkAnalyst如何对Microarray/RNA-Seq表达谱的一站式分析。 打开该网站网址,点击Gene expression table,进入此分析模块。 首先,需要上传基因表达数据。这里有四个选项(从上往下): ...
Here we present a comprehensive, temporal program of gene expression data encompassing human PC differentiation (PCD) using RNA sequencing (RNA-seq). Our results reveal 6374 differentially expressed genes classified into four temporal gene expression patterns. A stringent pathway enrichment analysis of ...
从小鼠中分离出BMSCs,RNA-Seq分析发现老年BMSCs中迁移相关基因均下调。老年和年轻小鼠BMSCs成骨诱导7d后ALP活性和成骨诱导14d后钙矿物质沉积相当,表明BMSCs的成骨潜能在衰老过程中没有改变。再次从老年和年轻小鼠中分离BMSCs,Dil染色剂标记后体外增殖,胫骨内注射到另一批年轻小鼠中,三天后收获胫骨对成骨标志物Runx...