To begin, we collected gene expression signatures using droplet-based scRNA-Seq (Drop-Seq8) from unfractionated total bone marrow (TBM) samples as well as from weakly lineage-depleted bone marrow (DBM) Cd45/Ter119 dual-negative subsets in order to enrich for rarer cell types, from three diff...
Here we show that transfer learning鈥攖he branch of machine learning that concerns passing information from one domain to another鈥攃an be used to efficiently map bone marrow biology between species, using data obtained from single-cell RNA sequencing. We first trained a multiclass logistic ...
To alleviate such limitations, we built a statistical framework specifically designed to perform differential analysis in ICC. Our resulting R package, called scDiffCom, can be applied to any human or mouse single-cell RNA sequencing (scRNA-seq) dataset to analyze changes in ICC between two given...
Retraction notice to "Mouse bone marrow derived mesenchymal stem cells-secreted exosomal microRNA-125b-5p suppresses atherosclerotic plaque formation via inhibiting Map4k4" [Life Sci. 274 (2021) 119249]. F Lin,S Zhang,X Liu,... - 《Life Sciences》 被引量: 0发表: 2023年 Bone marrow ...
AIM: To investigate the effect of bone marrow-derived monocytes transfected with RNA of CT-26 (a cell line of mouse colon carcinoma) on antitumor immunity.METHODS: Mouse bone marrow-derived monocytes were incubated with mouse granulocyte macrophage colony stimulating factor (mGM-CSF) in vitro, and...
Also, since this was a single-cell RNAseq dataset, it enabled us to further refine the localization and characterization of dysfunctional T cell and monocytic lineages within these tumors. Populations enriched in the human tumors relative to surrounding bone marrow (involved) included T helper (Th...
SMART-Seq Mouse BCR (with UMIs) enables users to analyze B-cell receptor (BCR) repertoires from 10 ng–1 µg high-integrity (RIN>7) bulk total RNA samples from spleen, bone marrow, or peripheral blood mononuclear cells (PBMCs) or RNA extracted from mouse whole blood (RNA input ≥100...
Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment 2020, eLife Show abstract SCODE: An efficient regulatory network inference algorithm from single-cell RNA-Seq during differentiation 2017, Bioinformatics Show abstract AnimalTFDB 2.0: A reso...
1 A multi-omic analysis of primary mouse bone marrow neutrophils during aging and with respect to sex (continued). a, Table of significant ‘omic’ features as a function of organismal age and sex in our datasets based on DEseq2 (RNA-seq) or limma (Metabolomics and lipidomics), at FDR...
RNA was extracted from renin-expressing bone marrow cells isolated from Bac-Ren1c-GFP mice using a Becton-Dickinson Aria 2 cell sorter in the RPCI Flow Cytometry Core Facility. RNA from unsorted bone marrow and GFP+ cells was hybridized to MOE430 V2.0 oligonucleotide microarray GeneChip Arrays...