Gene expression profiles of bone marrow cells were obtained from RNA sequencing (RNA-seq) and differentially expressed genes (DEGs) were analyzed with an emphasis on hematopoiesis-related pathways. The results show that E'jiao promotes the proliferation of both BMNCs and HSCs, as well as the ...
However, since neutrophils are very RNA-poor, single-cell profiling of these cells has been technically challenging. Here, we generated a single-cell RNA-seq dataset of primary neutrophils from adult female and male mouse bone marrow. After stringent quality control, we found that previously ...
B.A. and N.R. performed them. R.Q. and Y.K. conducted the scRNA-seq analysis. R.G.-M. helped with the bulk RNA-seq analysis. J.H. provided infrastructure for access to human blood. I.K. and D.M.G. analyzed the results, prepared the figures and wrote the manuscript. All auth...
Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging 2021, Cell Stem Cell Citation Excerpt : We find that Igf1 expression is reduced in a bulk population of MSCs and that loss of Igf1 in Nestin-expressing cells can recapitulate middle-aged phenotypes. Altho...
Compared with traditional bulk sequencing technologies, single-cell technologies have advantages to evaluate cellular heterogeneity and investigate the evolution of cellular subpopulations from the tumor and microenvironment. Application of single-cell sequencing in Multiple Myeloma (MM) is especially beneficial...
同时e图与b图、bulk转录组结果相互印证。 Fig. 1: scRNA-seq analysis of the bone marrow microenvironment at a steady state 内皮细胞亚群 基于整合或者独立的两种方法,从骨髓脉管系统内鉴定出两个主要的亚群。为了获得每个亚群的生物标记,作者分别对每个亚群与其他类群进行差异表分析。窦状毛细血管的分支网络构成...
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The brief workflow of single-cell RNA sequencing for these orthopedic disorders Full size image ScRNA-seq has four major advantages over bulk RNA-seq in biological research. First, this approach can reveal cellular heterogeneity or specific subclusters; second, it can depict the trajectories of diff...
Single-cell RNA-seq data were accessed via the Gene Expression Omnibus (accession codeGSE116256). Bulk RNA- and ATAC-seq data generated during this study have been deposited in the Gene Expression Omnibus with the accession codeGSE240025.Source dataare provided with this paper. All of the othe...
[1]. Research on CSCs has been ongoing for decades. The central concept of CSCs indicates that limited numbers of tumor cells that are capable of self-renewal can fuel tumor growth. Like stem cells in the intestinal epithelium, skin and bone marrow, CSCs existing in the bulk of tumors ...