BONE marrowRNA sequencingNEUTROPHILSIMMUNE systemSEXUAL dimorphismMICEWidespread sex-dimorphism is observed in the mammalian immune system. Consistently, studies have reported sex differences in the transcriptome of immune cells at the bulk level, including neutrophils. Neutrophils are the most abundant cell...
Gene changes associated with the dietary-induced loss of bone mass inEsr1Nkx2.1-crefemale mice were captured by profiling microdissected ARC and other tissues. Bulk RNA-seq of the ARC revealed a small set of upregulated differentially expressed genes (DEGs) encoding neuropeptides or secreted protein...
B.A. and N.R. performed them. R.Q. and Y.K. conducted the scRNA-seq analysis. R.G.-M. helped with the bulk RNA-seq analysis. J.H. provided infrastructure for access to human blood. I.K. and D.M.G. analyzed the results, prepared the figures and wrote the manuscript. All aut...
Single-cell RNA-seq data were accessed via the Gene Expression Omnibus (accession codeGSE116256). Bulk RNA- and ATAC-seq data generated during this study have been deposited in the Gene Expression Omnibus with the accession codeGSE240025.Source dataare provided with this paper. All of the othe...
Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging 2021, Cell Stem Cell Citation Excerpt : We find that Igf1 expression is reduced in a bulk population of MSCs and that loss of Igf1 in Nestin-expressing cells can recapitulate middle-aged phenotypes. Altho...
Bulk RNA sequencing (RNA-seq) was used to further explore the expression profile of BM EPCs, and in vitro coculture strategies were used to evaluate the abilities of BM EPCs to regulate haematopoiesis and immunity. Our aim was to provide a potential therapeutic strategy for MDS patients....
Table S2. Hematopoietic and stromal gene expression in CD44+ CD51– and CD51+ TNC populations from bulk RNA-Seq, Related to Figure 3. Z scores of gene expression values for hematopoietic- and stromal-associated across replicates for CD44+ CD51– TNCs and CD51+ TNCs. n = 3 mice per grou...
同时e图与b图、bulk转录组结果相互印证。 Fig. 1: scRNA-seq analysis of the bone marrow microenvironment at a steady state 内皮细胞亚群 基于整合或者独立的两种方法,从骨髓脉管系统内鉴定出两个主要的亚群。为了获得每个亚群的生物标记,作者分别对每个亚群与其他类群进行差异表分析。窦状毛细血管的分支网络构...
These include: (1) simple isolation protocols from small volumes of bone marrow (BM) aspirates, (2) ease of in vitro proliferation/expansion, (3) standard and defined culture medium, (4) low senescence through multiple passages, (5) ability to be gene modified, and (6) distinct in vivo...
Compared with traditional bulk sequencing technologies, single-cell technologies have advantages to evaluate cellular heterogeneity and investigate the evolution of cellular subpopulations from the tumor and microenvironment. Application of single-cell sequencing in Multiple Myeloma (MM) is especially beneficial...