参考文献:A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia 目标:利用文章中描述的工具和方法,复现文中主要的scRNA-seq分析图表 前言 最有效的学习方法就是在实践中学习,今天开始要尝试复现的文章是A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia,该文章于2019...
Current single-cell RNA sequencing (scRNA-seq) technology has revealed heterogeneity and sketchy differential hierarchy of skeletal cells. Skeletal stem and progenitor cells (SSPCs) are located upstream of the hierarchy and differentiate into chondrocytes, osteoblasts, osteocytes, and bone marrow ...
Average UMI expression in myeloid and progenitor clusters from bone marrow scRNA-seq, segregated by treatment. Supplementary Table 3 Clinical metadata for treatment-naive patients with NSCLC profiled by plasma Olink analysis. Supplementary Table 4 Olink values for plasma from treatment-naive patients wit...
Fig. 1: scRNA-seq analysis of the bone marrow microenvironment at a steady state 内皮细胞亚群 基于整合或者独立的两种方法,从骨髓脉管系统内鉴定出两个主要的亚群。为了获得每个亚群的生物标记,作者分别对每个亚群与其他类群进行差异表分析。窦状毛细血管的分支网络构成骨髓中的大部分血管,而动脉包含相对较少的...
In this study, we constructed a single-cell landscape of the OP microenvironment using scRNA-seq data from human femoral heads. We identified several immune cell subpopulations potentially involved in OP formation and pinpointed the transcription factors (TFs) regulating these subpopulations. These ...
Bone marrow mesenchymal stromal/stem cells (MSCs) are a heterogeneous population that can self-renew and generate stroma, cartilage, fat, and bone. Although a significant progress has been made toward recognizing about the phenotypic characteristics of M
Single-cell RNA sequencing (scRNA-seq) has been previously utilized to understand this hematopoietic malignancy in both tumor and immune populations in MM (Ledergor G. et al., 2018, Zavidij, O. et al., 2020). Mass cytometry (CyTOF) has also been used to identify the expansion of novel...
Iron overload exerts a significant impact on the bone mineralization process; moreover, the death of osteoblasts is significantly increased by iron overload, resulting in a decrease in mineralization capacity. When the iron load is excessive, iron accumulates primarily in the bone marrow, along with...
Our research aims to meticulously unravel these molecular dynamics, providing crucial insights into the biological processes that promote tumor cell resilience and expansion within the bone marrow niche. This exploration is imperative to identify prospective molecular and cellular therapeutic targets, ...
(scRNA-seq) and spatial transcriptome sequencing have been gradually employed to decipher the profile of CSCs[2]. Regarding functional properties, increasing evidence suggests that CSCs might be involved in multiple steps of tumor initiation, progression, and recurrence. In addition, CSCs are also ...