RNA-Seq Based Transcriptome Analysis of Endothelial Differentiation of Bone Marrow Mesenchymal Stem CellsGene expressionEndothelial differentiationStem cellsEndothelial cellsObjective: The aim was to identify the change in gene expression between mesenchymal stem cells (MSCs) and induced endothelial cells (ECs...
Mesenchymal stem cells (MSCs) are a common kind of multipotent cell in vivo, but their heterogeneity limits their further applications. To identify MSC subpopulations and clarify their relationships, we performed cell mapping of bone-marrow-derived MSCs through single-cell RNA (scRNA) sequencing. In...
In this study, we used RNA-seq to characterize the genome-wide expression portrait of human MSCs derived from bone marrow (BMs), adipose tissue (AMs), and palatine tonsil (TMs). Further, we determined the phenotypes of BMs, AMs, and TMs to verify common MSC surface markers as well as ...
Table S1. Analysis of the RNA-Seq Data from Nestin+ BMSCs Obtained from Leukemic iMLL-AF9 and Normal Mice, Related to Figure 4 Table S2. Analysis of the RNA-Seq Data from Leukemic BM lin− ckitlo Cells Obtained from Mice with/without Nestin+ Cell Depletion, Related to Figure 4 Tabl...
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目标:利用文章中描述的工具和方法,复现文中主要的scRNA-seq分析图表 前言 最有效的学习方法就是在实践中学习,今天开始要尝试复现的文章是A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia,该文章于2019年发表在Cell杂志上。文章的分析代码并未开源,因此只能根据原文描述来尽量复现其结果。
Gene expression profiling is a useful tool for cancer diagnosis and basic research. A major limitation is that, even during short-term storage of native specimens of peripheral blood or bone marrow (BM) and/or RNA isolation, significant changes of gene expression pattern can occur because of gen...
Using RNA sequencing analysis in blood, we have found for the first time a corresponding signature for a decreased (NR) or enhanced (R) bone marrow stem cell response upon ischemic/inflammatory response to CABG. Gene expression patterns are characteristic for a RvsNR preoperative steady-state and...
Extended Data Fig. 1 RNA-seq analysis of the bone marrow microenvironment populations Extended Data Fig. 2 Analysis of VE-Cad+, LEPR+ and COL2.3+ populations Extended Data Fig. 3 Characterization of VE-Cad+ subpopulations Extended Data Fig. 4 Characterization of perivascular LEPR+ subpopulations ...
Global characterization of megakaryocytes in bone marrow, peripheral blood, and cord blood by single-cell RNA sequencing Article01 June 2022 Introduction Blood irrigates all organs, supplying oxygen and nutrients to the cells of the body while collecting byproducts of cell metabolism, including lipids...