伯远医学拥有一流的技术团队、丰富的项目经验，提供专业RNA Pull-down技术服务，适用于RNA与蛋白结合的领域研究，欢迎大家前来咨询RNA Pull-down实验。参考文献 Barnes C, Kanhere A. Identification of RNA–protein interactions through in vitro rna Pull-down assays[J]. Polycomb Group Proteins: Methods and Prot...
CircPVT1 is actively induced by HuR.a Silver staining of biotinylated circPVT1-associated proteins;b Western blotting of HuR from circPVT1 pull-down assays;c RIP confirmed the relationship between circPVT1 and HuR in H520 cells.GAPDH mRNA was used as a non-HuR target control. 本文来自金开瑞...
"Identification of RNA–protein interactions through in vitro rna pull-down assays." Polycomb Group Proteins: Methods and Protocols (2016): 99-113. 4. Hellman, Lance M., and Michael G. Fried. "Electrophoretic mobility shift assay (EMSA) for detecting protein–nucleic acid interactions." Nature...
DNA Pull Down: A Comparison While RNA Pull Down focuses on RNA-protein interactions, DNA pull down assays are designed to study DNA-protein interactions. DNA pull down typically involves immobilizing DNA fragments onto a solid support and incubating them with protein extracts to capture DNA-binding...
RNA pull down实验是用生物素标记体外转录的靶RNA分子,再用链霉亲和素纯化出与靶RNA结合的蛋白复合体,洗脱得到RNA结合蛋白质。 技术应用 靶RNA结合蛋白的验证或筛选。 技术流程 服务信息 参考文献 1.Identification of RNA-Protein Interactions Through In Vitro RNA Pull-Down Assays. Methods MolBiol 1480:99-113...
1.Identification of RNA-Protein Interactions Through In Vitro RNA Pull-Down Assays. Methods MolBiol 1480:99-113(2016). 2.The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1. Sci Rep 6:32189(2016). ...
1.Identification of RNA-Protein Interactions Through In Vitro RNA Pull-Down Assays. Methods MolBiol 1480:99-113(2016). 2.The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1. Sci Rep 6:32189 (2016). ...
RNA pull down实验显示,hsa_circ_0001573与GNB4蛋白结合,FISH-IF表明hsa_circ_0001573与GNB4共定位于乳腺癌细胞中,且与GNB4相互作用能促进c-myc的表达。结论:环状RNA hsa_circ_0001573在乳腺癌中高表达,敲低hsa_circ_0001573对细胞增...
Consistently, RNA pulldown assay showed a stronger binding of SRSF7 to the edited intron 8 RNA probe than the wild-type probe; while hnRNPK, which was not predicted to differentially bind to edited CCDC15 and included as a negative control, showed a similar binding affinity to both wild-...
G Western blot of the AURKA proteins from sense and antisense SOCS2-AS1 pull-down assays. H RNA immunoprecipitation experiments were performed using anti-AURKA antibody, and qPCR was used to detect SOCS2-AS1. MEG3 was used as a negative control. I Western blot of AURKA in samples pulled ...