RIP、RNA pull-down和MeRIP等实验技术是我们深入研究RNA和蛋白质相互作用的关键工具。RIP用于选择性地富集与特定RNA相互作用的蛋白质,RNA pull-down通过RNA探针捕获相互作用蛋白质,而MeRIP则用于甲基化RNA的研究。下面就让我们详细了解一下—— RIP RIP,全称为RNA Binding Protein Immunoprecipitation Assay,RNA 结合蛋白...
伯远医学拥有一流的技术团队、丰富的项目经验，提供专业RNA Pull-down技术服务，适用于RNA与蛋白结合的领域研究，欢迎大家前来咨询RNA Pull-down实验。参考文献 Barnes C, Kanhere A. Identification of RNA–protein interactions through in vitro rna Pull-down assays[J]. Polycomb Group Proteins: Methods and Prot...
伯远医学拥有一流的技术团队、丰富的项目经验,提供专业RNA Pull-down技术服务,适用于RNA与蛋白结合的领域研究,欢迎大家前来咨询RNA Pull-down实验。 参考文献 Barnes C, Kanhere A. Identification of RNA–protein interactions through in vitro rna Pull-down assays[J]. Polycomb Group Proteins: Methods and Proto...
Here we describe an RNA-protein pull-down assay, which enables the identification of proteins that interact with an RNA under study. As an example we describe pull-down of proteins interacting with ncRNA XIST, which assists in the recruitment of the polycomb-repressive complex-2 (PRC2) and ...
DNA Pull Down: A Comparison While RNA Pull Down focuses on RNA-protein interactions, DNA pull down assays are designed to study DNA-protein interactions. DNA pull down typically involves immobilizing DNA fragments onto a solid support and incubating them with protein extracts to capture DNA-binding...
1.Identification of RNA-Protein Interactions Through In Vitro RNA Pull-Down Assays. Methods MolBiol 1480:99-113(2016). 2.The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1. Sci Rep 6:32189(2016). ...
1.Identification of RNA-Protein Interactions Through In Vitro RNA Pull-Down Assays. Methods MolBiol 1480:99-113(2016). 2.The long noncoding RNA ASNR regulates degradation of Bcl-2 mRNA through its interaction with AUF1. Sci Rep 6:32189 (2016). ...
RNA pull down实验是用生物素标记体外转录的靶RNA分子,再用链霉亲和素纯化出与靶RNA结合的蛋白复合体,洗脱得到RNA结合蛋白质。 技术应用 靶RNA结合蛋白的验证或筛选。 技术流程 服务信息 参考文献 1.Identification of RNA-Protein Interactions Through In Vitro RNA Pull-Down Assays. Methods MolBiol 1480:99-113...
Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome.Affinity purificationCaulobacterEpitope-tagged protein... S Hardwick,B Luisi,M Marques - 《Methods in Molecular Biology》 被引量: 0发表: 2021年 RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA desta...
RNA pull-down and RNA immunoprecipitation (RIP) Both full-length and shorter probes specific to LOC101928222, as well as negative control probes, were generated by Biotech. RNA pull-down assays were conducted with the Pierce Magnetic RNA-Protein Pull-down Kit (Thermo Fisher Scientific) according ...