RNA pull-down assay Long Noncoding RNA as Modular Scaffold of Histone Modification Complexes Miao-Chih Tsai, et al.RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with...
RNA pull-down assay Long Noncoding RNA as Modular Scaffold of Histone Modification Complexes Miao-Chih Tsai, et al.RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with...
RNApull-downassayLongNoncodingRNAasModularScaffoldofHistoneModificationComplexesMiao-ChihTsai,etal.RNApull-downassayusingnuclearextract1.Biotin-labe..
At BOC Sciences, we offer cutting-edge RNA pull down assay services to facilitate the study of RNA-protein interactions with precision and reliability. What is RNA Pull Down? Currently, methods for studying RNA-protein interactions can be broadly categorized into two types. One focuses on the ...
Mechanistically, by using biotinylated-circHIPK3 probe to perform RNA pull-down assay in CRC cells, we identified miR-7 was the only one microRNA that... Kaixuan,Zeng,Xiaoxiang,... - 《Cell Death & Disease》 被引量: 36发表: 2018年 Visible-light induced photoelectrochemical biosensor for th...
in thelncRNA-HEIHoverexpression and was not correlated with the survival rate of HCC patients (Supplementary Fig.5e,f). To determine whether miR-194-5p binds tolncRNA-HEIH, we performed an RNA pull-down assay using ASOs and found that miR-194-5p was enriched in thelncRNA-HEIHpull-down...
Furthermore, using bioinformatics analysis, luciferase reporter, RNA immunoprecipitation, and RNA pull-down assay, we identified that KCNQ1OT1 functions as a competing endogenous RNA (ceRNA) by sponging miR-34c-5p, which inhibited ALDOA expression by directly targeting its 3ʹUTR. Taken together,...
[141]. Wang et al. demonstrated how SNHG6 can promote tumor progression in HCC through a similar post-transcriptional mechanism. The study showed that silencing SNHG5 resulted in elevated levels of SETD7 and LZTFL1 mRNA in HCC cell lines. The RNA pull-down assay and RIP analysis ...
Meanwhile, RNA pull-down assay using a biotin labeled RUNX3 probe could enrich the expression of Suv39H1 protein, indicating the interaction between RUNX3 and Suv39H1 (Fig. 6J). Following this, ChIP assay verified that the RUNX3 antibody had indeed pulled down the Suv39H1 promoter (Fig. ...
Yeast two‐hybrid assay and BiFC assay RT‐qPCR assays Y1H and LUC reporter assays Bimolecular fluorescence complementation、pull-down assays、CoIP 03 主要结果 3.1 lncRNA MtCIR2的表达是由低温诱导的 我们之前的研究确定了一种冷响应lncRNA MtCIR1。为了寻找更具冷响应性的lncRNA,并测试它们是否通过与Mt...