At BOC Sciences, we offer cutting-edge RNA pull down assay services to facilitate the study of RNA-protein interactions with precision and reliability. What is RNA Pull Down? Currently, methods for studying RNA-protein interactions can be broadly categorized into two types. One focuses on the ...
十、RNA Pulldown 使用体外转录法标记生物素RNA探针,然后与胞浆蛋白提取液孵育,形成RNA-蛋白质复合物,该复合物可与链霉亲和素标记的磁珠结合,从而与孵育液中的其他成分分离。复合物洗脱后,通过WB或MS来检测。 实验操作视频链接:/video/57786/biotin-based-pulldown-assay-to-validate-mrna-targets-cellular©...
十、RNA Pulldown 使用体外转录法标记生物素RNA探针,然后与胞浆蛋白提取液孵育,形成RNA-蛋白质复合物,该复合物可与链霉亲和素标记的磁珠结合,从而与孵育液中的其他成分分离。复合物洗脱后,通过WB或MS来检测。 实验操作视频链接:https://www./video/57786/biotin-based-pulldown-assay-to-validate-mrna-targets-ce...
RNA pull-down assay Long Noncoding RNA as Modular Scaffold of Histone Modification Complexes Miao-Chih Tsai, et al.RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with...
RNA pull-down assay Long Noncoding RNA as Modular Scaffold of Histone Modification Complexes Miao-Chih Tsai, et al.RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with...
3 评论次数: 0 文档热度: 文档分类: 行业资料--石油、天然气工业 文档标签: RNA8 系统标签: rnaassayresuspendedbiotinylatedcorestpelleted RNApull-downassayLongNoncodingRNAasModularScaffoldofHistoneModificationComplexesMiao-ChihTsai,etal.RNApull-downassayusingnuclearextract1.Biotin-labeledRNAswereinvitrotranscribed...
This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.Keywords: miRNA, Biotin-pulldown, miRNA-mRNA interaction, Transfection, Biotin labelling...
Performing the Labeled microRNA pull-down (LAMP)assay system: an experimental approach for high-throughput identification ofmicroRNA-target mRNAs. HSU R J,TSAI H J. Methods in Molecular Biology . 2011Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification...
Further applications following the pull-down assay will be based on the research needs. This protocol does not necessarily require a transfection step. However, it is recommended to overexpress the circular RNA plasmid along with the vector plasmid control to achieve better comparison for later ...
(Fig.5D). RNA pulldown assay exhibited that there was no obvious binding ability between IRF1 mRNA and IGF2BP3 protein in GC cells (Fig.5E). Moreover, IGF2BP3 protein and IRF1 mRNA mainly distributed in the cytoplasm and nucleus respectively by subcellular fractionation (Fig.5F, Fig.S6)...