GST pull down(GST融合蛋白沉降技术)是体外检测蛋白相互作用的常用方法,一般来说,GST融合蛋白pull down方法用于两个方面:一是鉴定能与已知融合蛋白相互作用的未知蛋白质;二是鉴定两个已知蛋白质之间是否存在相互作用。为排除假阳性所设的一组对照实验是在没有诱饵蛋白存在的条件下,将样品结合到GST上。该对照可以是表...
RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with RNase-free DNase I (Promega) and purified with RNeasy Mini Kit (QIAGEN).2. Biotin-HRP Northern blot was ...
RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with RNase-free DNase I (Promega) and purified with RNeasy Mini Kit (QIAGEN).2. Biotin-HRP Northern blot was ...
除RIP和RNA pull Down以外，凝胶迁移或电泳迁移率检测(Electrophoretic Mobility Shift Assay, EMSA)，也称为凝胶阻滞实验或DNA结合实验，是一种广泛应用于分子生物学的研究技术，也可以用于研究蛋白质（尤其是转录因子）与DNA或RNA之间的相互作用。基本原理 EMSA的基本原理是利用凝胶电泳技术来观察蛋白质与DNA或RNA形成...
3 评论次数: 0 文档热度: 文档分类: 行业资料--石油、天然气工业 文档标签: RNA8 系统标签: rnaassayresuspendedbiotinylatedcorestpelleted RNApull-downassayLongNoncodingRNAasModularScaffoldofHistoneModificationComplexesMiao-ChihTsai,etal.RNApull-downassayusingnuclearextract1.Biotin-labeledRNAswereinvitrotranscribed...
究竟会有什么效应,从而更加详细的揭示出DNA与蛋白质...六、拉下实验(Pull-down assay) 拉下实验又叫做...例如RIP(其实就是用ChIP的方法研究细胞内蛋白与RNA...
Figure 2. Flowchart of BOC Sciences RNA pull down assay service. a: In the process of constructing RNA overexpression plasmids, firstly, the RNA of interest is screened and identified through various methods, and the corresponding RNA nucleotide sequence is retrieved from databases. RACE amplification...
1)、RNA Pull-down———In Vitro体外通过RNA探针捕获目的蛋白或其他候选蛋白,获取蛋白后进行WB检测或MS质谱鉴定。也是经典方法。 2)、RNA-EMSA———RNA电泳凝胶迁移阻滞实验(Electrophoretic Mobility Shift Assay),In Vitro体外通过RNA探针检测目的蛋白,反向验证了RIP。是经典方法。
Mechanistically, by using biotinylated-circHIPK3 probe to perform RNA pull-down assay in CRC cells, we identified miR-7 was the only one microRNA that... Kaixuan,Zeng,Xiaoxiang,... - 《Cell Death & Disease》 被引量: 36发表: 2018年 Visible-light induced photoelectrochemical biosensor for th...