你可以看看 GST-tag ，his- tag，等等pull down assay 的实验原理，虽然这些是针对蛋白的，但是原理一样，很简单的，记得做好control就行了。
等等pull down assay 的实验原理，虽然这些是针对蛋白的，但是原属理一样，很简单的，记得做好control就...
如题 DNA-affinity pull-down assay，翻译成中文，标准的翻译是什么？谢谢！
elegans telomeric sequence, we employed a DNA pulldown assay (Supplementary Fig. 1a, b) previously used to successfully identify telomeric proteins in other species15,16,39,40. We incubated concatenated, biotinylated DNA oligonucleotides consisting of either the telomeric sequence of C. elegans (TT...
DNA-binding proteinDNA-conjugated beadsDNA–protein interactions (DPIs)Pull-down assayDNA-protein interactions (DPIs) are critical to all living organisms, particularly in the regulation of gene expression, replication, packing, recombination, and DNA repair, as well as RNA transport and translation. ...
Thus, the promoter pull-down assay was developed to fill this void. This method uses DNA as bait to capture proteins that bind to a specific promoter, such as transcription factors, from cellular lysates. Coupled with other experiments, the promoter pull-down assay vastly improves the repertoire...
Here we describe an effective approach to identify potential known/novel DNA or RNA sensors: a pull-down assay using DNA/RNA-conjugated beads with a customized competition strategy, which conferred a more effective and efficient way to determine the interaction between DNA/RNA and the sensor ...
The DPC pull-down assay is a high-stringency plasmid pull-down protocol to specifically isolate DNA crosslinked HpaII from extract (Larsen et al., 2018). Briefly, Streptavidin-coupled magnetic beads (Dynabeads M-280, Invitrogen; 10 μL per pull-down) were washed twice with Wash buffer #1 (...
A Strep-Tactin pulldown assay was carried out as described in ref. 56. Briefly, 293FT cells were transfected with plasmid harbouring Strep-Myc-tagged ubiquitin using X-tremeGENE HP (Roche) according to the manufacturer’s instructions. Cells were lysed in denaturing buffer (20 mM Tris, pH...
In vitro pull-down assays To express proteins in a cell-free system, 2HA-CDF2, PIF4-6Myc and the truncated DNA fragments were amplified by overlap PCR, then cloned into EcoRI-digested pTnT vector with the In-Fusion HD Cloning Kit (Takara Bio). For each construct (2HA-CDF2, PIF4-...