Pull Down Assay 一、GST Pull Down Pull down技术的基本原理是将一种蛋白质固定于某种基质上(如Sepharose),但细胞抽提液经过该基质时,可与该固定蛋白相互作用的配体蛋白被吸附,而没有被吸附的“杂质”则随洗脱液流出。被吸附的蛋白可以通过改变洗脱液或洗脱条件而回收下来。通过pull down技术可以确定已知的蛋白与钓...
RIP用于选择性地富集与特定RNA相互作用的蛋白质,RNA pull-down通过RNA探针捕获相互作用蛋白质,而MeRIP则用于甲基化RNA的研究。下面就让我们详细了解一下—— RIP RIP,全称为RNA Binding Protein Immunoprecipitation Assay,RNA 结合蛋白免疫沉淀,其主要目的是富集RNA分子和与之相互作用的蛋白质,以便进一步分析这些相互作用...
Pull Down Assay GST Pull Down Pull down技术的基本原理是 将一种蛋白质固定于某种基质上(如Sepharose),但细胞抽提液经过该基质时,可与该固定蛋白相互作用的配体蛋白被吸附,而没有被吸附的“杂质”则随洗脱液流出。 被吸附的蛋白可以通过改变洗脱液或洗脱条件而回收下来。通过pull down技术可以确定已知的蛋白与钓...
一、RNA-Protein研究 1. RNA Pull Down Assay(1) 为了鉴定特定的RNA分子是否与某一种特定的蛋白结合,研究人员根据DNA-Protein实验中DNA Pull down的实验方法进行改进,使其在RNA上实现。 技术步骤如下: (1)设计末端携带T7 RNA Promoter的引物扩增DNA片段; (2)利用T7 RNA polymerase 进行转录,转录过程添加携带生...
Pull Down Assay GST Pull Down Pull down技术的基本原理是 将一种蛋白质固定于某种基质上(如Sepharose),但细胞抽提液经过该基质时,可与该固定蛋白相互作用的配体蛋白被吸附,而没有被吸附的“杂质”则随洗脱液流出。 被吸附的蛋白可以通过改变洗脱液或洗脱条件而回收下来。通过pull down技术可以确定已知的蛋白与钓...
At BOC Sciences, we offer cutting-edge RNA pull down assay services to facilitate the study of RNA-protein interactions with precision and reliability. What is RNA Pull Down? Currently, methods for studying RNA-protein interactions can be broadly categorized into two types. One focuses on the ...
Here we describe an RNA-protein pull-down assay, which enables the identification of proteins that interact with an RNA under study. As an example we describe pull-down of proteins interacting with ncRNA XIST, which assists in the recruitment of the polycomb-repressive complex-2 (PRC2) and ...
RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with RNase-free DNase I (Promega) and purified with RNeasy Mini Kit (QIAGEN).2. Biotin-HRP Northern blot was ...
RNA pull-down assay using nuclear extract 1. Biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase (Promega), treated with RNase-free DNase I (Promega) and purified with RNeasy Mini Kit (QIAGEN).2. Biotin-HRP Northern blot was ...
RNA-Protein pull down INSTRUCTIONS Pierce? Magnetic RNA-Protein Pull-Down Kit 20164 Number 20164 2509.0 Description Pierce Magnetic RNA-Protein Pull-Down Kit, contains sufficient reagents for 20 desthiobiotinylation reactions with 50pmol of RNA and 20 pull-down reactions using 50pmol of labeled RNA...