(E) DNA pull-down assays using Nanog wild-type or ERRE mutated probe, which also contains a neighboring Oct–Sox site (van den Berg et al. 2008), and cell extracts from COS-1 transfected with Ncoa3, Oct4, and Sox2. 第五部分:DNA Pull-down试剂盒组分清单(共计15个组分,均可单独购买) ...
其中,RNA/DNA pull down是研究体外条件下RNA或DNA与蛋白互作的重要技术。该技术通过体外转录目的RNA的一部分或全长,用生物素标记转录产物,再与链霉亲和素磁珠孵育使之固定在磁珠上,用以“pull down”互作蛋白,纯化的产物蛋白经特定抗体的western-blot实验,可验证某种蛋白与目的RNA/DNA是否有相互作用;纯化的蛋白经质...
Many laboratory techniques have been developed to study the complex interactions of proteins with DNA, such as chromatin immunoprecipitation (ChIP) assays, DNA electrophoretic mobility shift assay (EMSA), and oligonucleotide pull-down assays. Here we describe an effective approach to identify potential ...
DNA pulldown试剂盒利用预先使用BSA、寡核苷酸随机引物封闭磁珠,降低了磁珠与蛋白、基因组DNA的非特异性结合。DNA pulldown技术是一种用于研究DNA与蛋白质互作的技术,通常用来分析目标基因调控区域相结合的转录因子及其他调控蛋白。针对目标区域设计脱硫生物素标记的特异性DNA探针,并与细胞核提取物孵育。 通过偶联磁珠,经...
(E) DNA pull-down assays using Nanog wild-type or ERRE mutated probe, which also contains a neighboring Oct–Sox site (van den Berg et al. 2008), and cell extracts from COS-1 transfected with Ncoa3, Oct4, and Sox2. 第五部分:DNA Pull-down试剂盒组分清单 (共计15个组分,均可单独购买...
Optimization of Biotinylated RNA or DNA Pull-Down Assays for Detection of Binding Proteins: Examples of IRP1, IRP2, HuR, AUF1, and Nrf2 2023, International Journal of Molecular Sciences A DNA Pull-Down Assay with Diversity Forms of Competitor for Detecting or Evaluating Protein–DNA Interactions ...
Pull-down assays Pull-down assays were used to confirm chosen protein−protein interactions. Equimolar amounts of all proteins were used at 5 nM per pull-down. Recombinant FPG-6xHis or 6xHis-MPG bait proteins were first bound to nickel-nitrilotriacetic acid (NTA) coupled resin in a Tris...
DNA pull-down assays showed that the assembly of Ku70/Ku80 and DNA-PKcs is not affected by p18-Cyclin E. However, the recruitment of XRCC4, Ligase IV, and the recently identified accessory factor XLF was greatly impaired. It appears that p18-Cyclin E profoundly affects NHEJ which is ...
Pulldowns were performed with embryo extracts of TEBP-1::3xFLAG or TEBP-2::GFP animals. Uncropped blots in Source Data. N = 3 biologically independent experiments with similar results, g, h Fluorescence polarization assays of 4 µM to 4 nM purified TEBP-1-His5 and TEBP-2-...
Here we present the detection of a synthetic DNA sequence from Herpes Simplex Virus-1 within swine cerebrospinal fluid (CSF), using a sandwich-like, magnetic nanoparticle pull-down assay. Magnetic nanoparticles and fluorescent polystyrene nanoparticles were both modified with DNA probes, able to ...