Introduction to pull-down assays The pull-down assay is anin vitromethod used to determine a physical interaction between two or more proteins. Pull-down assays are useful for both confirming the existence of a protein–protein interaction predicted b...
1、两个非同一定位的蛋白可能因电荷或是强亲和力的原因造成假阳性，需用其他方法再次验证，例如Co-IP等；2、验证互作是在试管中进行的生化反应，不能够完全反映细胞内蛋白真实互作的状态；3、融合表达的GST标签，肽链较长，可能会改变原目的蛋白的原有的折叠结构。实验信息 实验过程中客户可以随时登入管理系统查看项目...
Pull-down assays are a form of affinity purification and are very similar to immunoprecipitation, except that a "bait" protein is used instead of an antibody. Affinity chromatography (i.e., affinity purification) methodologies greatly enhance the speed and efficiency of protein purification and ...
CircPVT1 is actively induced by HuR.a Silver staining of biotinylated circPVT1-associated proteins;b Western blotting of HuR from circPVT1 pull-down assays;c RIP confirmed the relationship between circPVT1 and HuR in H520 cells.GAPDH mRNA was used as a non-HuR target control. 本文来自金开瑞...
Introduction to pull-down assays The pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. Pull-down assays are useful for both confirming the existence of a protein–protein interaction predicte...
Barnes C, Kanhere A. Identification of RNA–protein interactions through in vitro rna Pull-down assays[J]. Polycomb Group Proteins: Methods and Protocols, 2016: 99-113.Dou R, Han L, Yang C, et al. Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through ...
Identification of RNA–protein interactions through in vitro rna Pull-down assays[J]. Polycomb Group Proteins: Methods and Protocols, 2016: 99-113. Dou R, Han L, Yang C, et al. Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial‐...
Determining protein partners is an essential step toward understanding protein function and identifying relevant biological pathways. Many methods exist for investigating ...
Introduction to Pull-Down Assays The pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. Pull-down assays are useful for both confirming the existence of a protein-protein interaction predicted by other research techniques (e.g., co-immun...
. .12Pull-down assays overviewPull-down assays probe interactions between a protein of interest that is expressed as a fusionprotein (e.g., bait) and the potential interacting partners (prey).In a pull-down assay one protein partner is expressed as a fusion protein (e.g., bait protein) ...