RNA pull-down的主要目的是通过特定的RNA分子(通常是已知的非编码RNA或mRNA的片段)来富集与之相互作用的蛋白质,以便进一步研究这些相互作用的性质和功能。RNA pull-down的基本步骤如下:① RNA标记:首先,标记目标RNA分子(通常是合成的in vitro转录RNA),通常使用生物素(biotin)标记。这种标记的RNA片段可以被用作“鱼饵...
伯远医学拥有一流的技术团队、丰富的项目经验，提供专业RNA Pull-down技术服务，适用于RNA与蛋白结合的领域研究，欢迎大家前来咨询RNA Pull-down实验。参考文献 Barnes C, Kanhere A. Identification of RNA–protein interactions through in vitro rna Pull-down assays[J]. Polycomb Group Proteins: Methods and Prot...
DNA Pull Down: A Comparison While RNA Pull Down focuses on RNA-protein interactions, DNA pull down assays are designed to study DNA-protein interactions. DNA pull down typically involves immobilizing DNA fragments onto a solid support and incubating them with protein extracts to capture DNA-binding...
"Identification of RNA–protein interactions through in vitro rna pull-down assays." Polycomb Group Proteins: Methods and Protocols (2016): 99-113. 4. Hellman, Lance M., and Michael G. Fried. "Electrophoretic mobility shift assay (EMSA) for detecting protein–nucleic acid interactions." Nature...
核酸蛋白互作:RNA/DNA pull down,CHIRP 技术简介 核酸与蛋白互作不仅是生物有机体展示其生命活动的基本形式之一,还是核酸和蛋白功能阐释的重要内容。常用于核酸与蛋白互作研究的技术包括:RNA pull down、DNA pull down、ChIRP、ChIP、reverse-ChIP、RIP等。
RNA pull down实验是用生物素标记体外转录的靶RNA分子,再用链霉亲和素纯化出与靶RNA结合的蛋白复合体,洗脱得到RNA结合蛋白质。 技术应用 靶RNA结合蛋白的验证或筛选。 技术流程 服务信息 参考文献 1.Identification of RNA-Protein Interactions Through In Vitro RNA Pull-Down Assays. Methods MolBiol 1480:99-113...
RNA pull down是体外研究RNA与蛋白互作的重要技术。该技术通过体外转录靶RNA的一部分或全长,用生物素标记转录产物,再与蛋白提取液孵育,形成RNA-蛋白质复合物。该复合物可与链霉亲和素磁珠结合,从而把靶RNA结合蛋白从蛋白提取液中分离出来。复合物从磁珠上洗脱下来,通过western blot实验检测特定的RNA结合蛋白是否与靶...
6a) was used in pulldown assays (Fig. 4g). The requirement for LOC and ‘active’ DHX15 helicase for the interaction of DHX15 with Wip1 suggested that LOC–DHX15 is a ribonucleoprotein (RNP) scaffold that sequesters Wip1. Interestingly, this RNP can only successfully trap Wip1 when the ...
RNA pull down实验显示,hsa_circ_0001573与GNB4蛋白结合,FISH-IF表明hsa_circ_0001573与GNB4共定位于乳腺癌细胞中,且与GNB4相互作用能促进c-myc的表达。结论:环状RNA hsa_circ_0001573在乳腺癌中高表达,敲低hsa_circ_0001573对细胞增...
Consistently, RNA pulldown assay showed a stronger binding of SRSF7 to the edited intron 8 RNA probe than the wild-type probe; while hnRNPK, which was not predicted to differentially bind to edited CCDC15 and included as a negative control, showed a similar binding affinity to both wild-...