RNA pull-down RNA pull-down的主要目的是通过特定的RNA分子(通常是已知的非编码RNA或mRNA的片段)来富集与之相互作用的蛋白质,以便进一步研究这些相互作用的性质和功能。RNA pull-down的基本步骤如下:① RNA标记:首先,标记目标RNA分子(通常是合成的in vitro转录RNA),通常使用生物素(biotin)标记。这种标记的RNA片段可...
The ceRNA regulatory mechanism of circNTRK2 was observed via dual-luciferase reporter, RIP and RNA pull-down assays. The mice xenograft models were constructed to confirm the oncogenicity of circNTRK2 in ESCC in vivo. Results: CircNTRK2 was highly expressed in ESCC tissues and cells. High ...
Up-regulation of lncRNA CASC9 promotes esophageal squamous cell carcinoma growth by negatively regulating PDCD4 expression through EZH2 Finally, the mechanism of CASC9 regulating PDCD4 was explored by RIP, RNA-protein pull down and ChIP assays. ESCC tissue microarrays showed that CASC9 ... Y Wu...
Dynabeads and Pierce magnetic beads and kits for chromatin IP (ChIP), RNA immunoprecipitation (RIP), and other protein and nucleic acid pull-down assays.
Dynabeads and Pierce magnetic beads and kits for chromatin IP (ChIP), RNA immunoprecipitation (RIP), and other protein and nucleic acid pull-down assays.
(A and B) Mapping the interaction between RIP1 and RIP3 using co-expression and His-tag pull down. Only lanes with positive interactions are labeled. Left panel: RIP1 was tagged with His-MBP. Right panel: RIP3 was tagged with His-MBP. (C) Strip plots from 3D NCOCA and NCACX experi...
· 分开回收smallRNA与large RNA(分离法) 不论通过哪一种方法,都可以比原来使用苯酚的方法更高效的回收miRNA。本试剂盒可以用于特定疾病、功能性相关的mRNA的鉴定,miRNA与靶mRNA的配对分析。 实验数据: 价格 *** 产地 MBL 规格 10 assays 库存量 100
However, an antagonistic TNF-R1 antibody had no effect on doxo- rubicin-induced cell death despite its ability to prevent TNF-R1-stimulated IκBα degradation, JNK activation, and cell death (Supplementary information, Figure S2E). In addition, knocking down TNF-R1 by shRNA did not reduce ...
7B). The reverse was also true as Flag-A20 was able to pull down kinase dead HA-RIPK1D138N (Fig. 7B). These results were further validated in the hepatocyte-derived cell line, Huh7 (Fig. S6, A and B). We confirmed the RIPK1-A20 interaction seen in our model was not due to ...
Immunoprecipitation and pull-down assays For co-immunoprecipitation of caspase-8 bound proteins, 1–2 × 107 cells were pre-stimulated with zVad (25 μM) 30 min prior to treatment with indicated substances. Caspase-8 was precipitated according to manufacturer’s instructions (Promega). Eq...