The pull-down assay is anin vitromethod used to determine a physical interaction between two or more proteins. Pull-down assays are useful for both confirming the existence of a protein–protein interaction predicted by other research techniques (e.g.,...
1、鉴定的蛋白是直接作用的蛋白，非间接作用的复合体；2、GST标记可把一些难溶蛋白变成可溶蛋白，使实验变得更易操作；3、GSH谷胱甘肽偶联球珠亲和力强，洗脱纯度高。拉下实验（Pull-down）的缺点 1、两个非同一定位的蛋白可能因电荷或是强亲和力的原因造成假阳性，需用其他方法再次验证，例如Co-IP等；2、验证互作...
Pull-down assays are a form of affinity purification and are very similar to immunoprecipitation, except that a "bait" protein is used instead of an antibody. Affinity chromatography (i.e., affinity purification) methodologies greatly enhance the speed and efficiency of protein purification and ...
The pull-down technique has become an invaluable tool for the life scientist interested in studying cellular pathways via protein–protein interactions. Page contents Introduction to pull-down assays Critical components of pull-down assays Pull-down meth...
CircPVT1 is actively induced by HuR.a Silver staining of biotinylated circPVT1-associated proteins;b Western blotting of HuR from circPVT1 pull-down assays;c RIP confirmed the relationship between circPVT1 and HuR in H520 cells.GAPDH mRNA was used as a non-HuR target control. 本文来自金开瑞...
Determining protein partners is an essential step toward understanding protein function and identifying relevant biological pathways. Many methods exist for investigating protein–protein interactions. The pull-down assay is an in vitro technique used to detect physical interactions between two or more prote...
Pull down实验步骤 一、Pull down实验特点 Pull down实验是一种广泛用于检测或确认多种蛋白质之间相互作用的体外方法[1]。该方法类似于免疫共沉淀实验,使用亲和配体捕获相互作用蛋白。这两种方法的不同之处在于,免疫共沉淀使用固定抗体来捕获蛋白质复合物,而Pull down方法使用纯化和标记的蛋白质作为“诱饵”来结合任何...
伯远医学拥有一流的技术团队、丰富的项目经验，提供专业RNA Pull-down技术服务，适用于RNA与蛋白结合的领域研究，欢迎大家前来咨询RNA Pull-down实验。参考文献 Barnes C, Kanhere A. Identification of RNA–protein interactions through in vitro rna Pull-down assays[J]. Polycomb Group Proteins: Methods and ...
. .12Pull-down assays overviewPull-down assays probe interactions between a protein of interest that is expressed as a fusionprotein (e.g., bait) and the potential interacting partners (prey).In a pull-down assay one protein partner is expressed as a fusion protein (e.g., bait protein) ...
The pull-down technique has become an invaluable tool for the life scientist interested in studying cellular pathways via protein-protein interactions. Introduction to Pull-Down Assays The pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. ...