Many laboratory techniques have been developed to study the complex interactions of proteins with DNA, such as chromatin immunoprecipitation (ChIP) assays, DNA electrophoretic mobility shift assay (EMSA), and oligonucleotide pull-down assays. Here we describe an effective approach to identify potential ...
中文名称: DNA Pull-down试剂盒 英文名称: DNA Pull-down Kit 品牌: Engibody 产地: 美国 产品类别: DNA和蛋白互作研究产品 货号: IF9606 用途范围: DNA和DNA蛋白的互作研究 规格: 20个反应 品名:DNA Pull-down Kit,货号:IF9606,品牌:Engibody订购及技术咨询:朱小鸥,13817407320(微信同号) NIH、...
常用于核酸与蛋白互作研究的技术包括:RNA pull down、DNA pull down、ChIRP、ChIP、reverse-ChIP、RIP等。 其中,RNA/DNA pull down是研究体外条件下RNA或DNA与蛋白互作的重要技术。该技术通过体外转录目的RNA的一部分或全长,用生物素标记转录产物,再与链霉亲和素磁珠孵育使之固定在磁珠上,用以“pull down”互作蛋...
DNA pulldown试剂盒利用预先使用BSA、寡核苷酸随机引物封闭磁珠,降低了磁珠与蛋白、基因组DNA的非特异性结合。DNA pulldown技术是一种用于研究DNA与蛋白质互作的技术,通常用来分析目标基因调控区域相结合的转录因子及其他调控蛋白。针对目标区域设计脱硫生物素标记的特异性DNA探针,并与细胞核提取物孵育。 通过偶联磁珠,经...
英文名称: Smart-DNA-Pull™ DNA Pull-down Kit 总访问: 98 国产/进口: 进口 半年访问: 98 产地/品牌: Engibody美国 产品类别: 细胞生物学试剂 规格: 20个反应 最后更新: 2024-9-27 货号: IF9606 CAS 号: 参考报价: 3500-3800 立即询价 电话咨询 ...
Optimization of Biotinylated RNA or DNA Pull-Down Assays for Detection of Binding Proteins: Examples of IRP1, IRP2, HuR, AUF1, and Nrf2 2023, International Journal of Molecular Sciences A DNA Pull-Down Assay with Diversity Forms of Competitor for Detecting or Evaluating Protein–DNA Interactions ...
Pulldowns were performed with embryo extracts of TEBP-1::3xFLAG or TEBP-2::GFP animals. Uncropped blots in Source Data. N = 3 biologically independent experiments with similar results, g, h Fluorescence polarization assays of 4 µM to 4 nM purified TEBP-1-His5 and TEBP-2-...
a, Schematic of NET-DNA pull-down assays. b, Mass spectrometry analysis identified CCDC25 as the cytoplasmic membrane protein from MDA-MB-231 cells pulled down by the biotinylated NET-DNA. c, Immunoblotting of the membrane proteins of MDA-MB-231 cells pulled down by biotinylated NET-DNA and...
random shearing, two amplicons reflecting the same target region but which originated from distinct input DNA molecules can be distinguished by the random ends of the amplicons. The ability to exclude duplicates makes copy number calculations more effective in whole genome and in pull-down assays.85...
Here, we coupled DNA affinity isolation with in-gel kinase assays to avidly pull down the activated NFAT and identify its associated protein kinases. We ... TTC Yang,Q Xiong,IA Graef,... - 《Molecular & Cellular Biology》 被引量: 132发表: 2005年 Akt promotes post-irradiation survival of...