The assay, referred to as proximity‐based labeling of membrane゛ssociated proteins (PLiMAP), is based on UV activation of a fluorescent lipid reporter, which in turn crosslinks with proteins bound to membranes and renders them fluorescent. 2020 Wiley Periodicals LLC. Basic Protocol 1: Synthesis...
Integrated Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Quantitative Proteomic Analysis Identifies Galectin-1 as a Potential Biomarker for Predicting Sora... Sorafenib has become the standard therapy for patients with adv...
To quantify the relative abundance of PL proteins, we prepared and analyzed Group I samples without p50 and Group II samples with p50 (Fig.2b). TurboID labeling was performed for 12 h by infiltration of biotin. Expression of tRFP-p50 was induced by the addition of estradiol at the same ...
文献阅读-1:Exploiting effector-host interactions using TurboID-based proximity labeling 1只小脑斧ya 总要坚持些什么吧1 人赞同了该文章 科学问题:Ustilago maydis效应子UmSee1是玉米感染期间叶片中肿瘤形成所必需的。UmSee1与玉米SGT1相互作用并阻断其在体内的磷酸化。然而,目前尚不清楚哪些宿主过程被UmSee1和Um...
Protocol Representative Results Discussion Acknowledgements Materials References English Explore More Videos Keywords TurboID Proximity Labeling Protein protein Interactions NLR Immune Receptors Nicotiana Benthamiana Agroinfiltration Biotin RIPA Lysis Buffer Desalting Column ...
Figure 1. Flowchart of the proximity labeling of SARS-CoV-2 proteins (A) The proteins encoded by the SARS-CoV-2 genome. (B) The workflow of the TurboID assay. To proximity label the proteins of SARS-CoV-2, all of the viral genes were cloned into mammalian expression vectors in frame ...
Ubiquitin-specific proximity labeling for the identification of E3 ligase substrates Hai-Tsang Huang Ryan J. Lumpkin William R. Sellers Nature Chemical Biology (2024) Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation Satoshi Yamanaka Hirotake Furihata Ta...
This assay measures the interaction of two target proteins in which one is tagged with the robust NanoLuc energy donor and the other contains the HaloTag for labeling with a fluorescent energy acceptor. When the tagged proteins of interest interact, energy is transferred from the bioluminescent ...
750 image data were obtained with a total size of 2.5 GB. The data were split into training and validation sets with a ratio of 70% for training and 30% for validation. The dataset was randomly shuffled during the splitting process. The data were labeled using the CVAT labeling tool [37...
Quinone Methide-Based Organophosphate Hydrolases Inhibitors: Trans Proximity Labelers versus Cis Labeling Activity-Based Probes Quinone methide (QM) chemistry is widely applied including in enzyme inhibitors. Typically, enzyme-mediated bond breaking releases a phenol product that re... A Dubovetskyi,KP...