Here, we used proximity labeling (PL) to identify the proteome proximal to N, which is an NLR that confers resistance to(TMV). Evaluation of different PL methods indicated that TurboID-based PL provides more efficient levels of biotinylation than BioID and BioID2 in plants. TurboID-based PL...
To determine whether the N NLR interacting proteins identified from TurboID-based proximity labeling function in theN-mediated resistance to TMV, we selected 10 proteins (Supplementary Table1) from the MS dataset for the subsequent genetic screening. For this, we selected proteins that spanned the r...
2019年7月19日,Nature Communications在线发表了中国农业大学张永亮课题组、加州大学戴维斯分校Savithramma P. Dinesh-Kumar课题组和爱荷华州立大学Justin W. Walley课题组合作题为“TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity”的研究论文。该研究首...
TurboID-based proximity la- beling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity. Nat Commun 10, 3252. © 植物学报 Chinese Bulletin of Botany 邝嘉怡等: 基于 TurboID 的植物蛋白邻近标记实验方法 593 Methods for TurboID-based Proximal Labeling in Plants Jiayi Kuang ...
Zhang, Y. et al. TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity.Nat. Commun.10, 3252 (2019). Larochelle, M., Bergeron, D., Arcand, B. & Bachand, F. Proximity-dependent biotinylation mediated by TurboID to identify protein-pr...
Enzyme-catalyzed proximity labeling has rapidly become a popular approach for studying the spatial localization and interactions of proteins in the cell. In this technique, a protein of interest is labeled with a promiscuous enzyme that adds a tag such as biotin to near-neighbor proteins, thereby ...
邻近标记(proximity labeling, PL)技术的基本原理是将一个具有连接酶活性的酶(目前植物中广泛应用的为TurboID)与目的蛋白融合, 在连接酶的催化作用下将小分子底物(如生物素(biotin))连接到与目的蛋白邻近的内源蛋白或RNA, 而后可通过富集被标记的蛋白或RNA分别进行质谱分析或RNA-Seq, 可鉴定目的蛋白邻近的蛋白或RNA...
Read this JoVE article on TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
文献阅读-1:Exploiting effector-host interactions using TurboID-based proximity labeling 1只小脑斧ya 总要坚持些什么吧1 人赞同了该文章 科学问题:Ustilago maydis效应子UmSee1是玉米感染期间叶片中肿瘤形成所必需的。UmSee1与玉米SGT1相互作用并阻断其在体内的磷酸化。然而,目前尚不清楚哪些宿主过程被UmSee1和Um...
TurboID-Based IRE1 Interactome Reveals Participants of the Endoplasmic Reticulum-Associated Protein Degradation Machinery in the Human Mast Cell Leukemia C... Therefore, the TurboID proximity labeling technique combined with MS analysis was applied. Gene Ontology and pathway enrichment analyses revealed tha...