To further confirm the interaction between N TIR domain andNbUBR7, we performed GST pull-down assays using recombinant proteins purified fromE. coli. Our results showed that GST-fusedNbUBR7 specifically pulled-down the GFP-fused TIR domain of N, but not the GFP-fused C-terminal region of th...
The Ti‐TAN plasmid toolbox for TurboID‐based proximity labeling assays in Nicotiana benthamianadoi:10.1111/jipb.13610Tan, HuangZhou, YuDinius, ErikLozano‐Durán, RosaJournal of Integrative Plant Biology
The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana Protein-protein interactions and the formation of protein complexes play a central role in the regulation of virtually all aspects of life.The identificati... T Huang,Z Yu,E Dinius,... - 《Journal ...
This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or clustered regularly interspaced short palindromic repeats (CRISPR)-based tagging, thus prohibiting the use of such assays in cell types that are difficult to transfect or transduce. We recently ...
The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana Protein-protein interactions and the formation of protein complexes play a central role in the regulation of virtually all aspects of life.The identificati... T Huang,Z Yu,E Dinius,... - 《Journal ...
Therefore, the TurboID proximity labeling technique combined with MS analysis was applied. Gene Ontology and pathway enrichment analyses revealed that the majority of the enriched proteins are involved in vesicle-mediated transport, protein stabilization, and ubiquitin-dependent ER-associated protein ...
Therefore, the TurboID proximity labeling technique combined with MS analysis was applied. Gene Ontology and pathway enrichment analyses revealed that the majority of the enriched proteins are involved in vesicle-mediated transport, protein stabilization, and ubiquitin-dependent ER-associated protein ...