In this study, we compared the activity and efficiency of different biotin ligases for proximity labeling in plants. Our results revealed that TurboID outperforms other biotin ligases in tagging biotin to the nearby proteins in plant cells at room temperature. We optimized the TurboID approach in t...
邻近标记(proximity labeling, PL)技术的基本原理是将一个具有连接酶活性的酶(目前植物中广泛应用的为TurboID)与目的蛋白融合, 在连接酶的催化作用下将小分子底物(如生物素(biotin))连接到与目的蛋白邻近的内源蛋白或RNA, 而后可通过富集被标记的蛋白或RNA分别进行质谱分析或RNA-Seq, 可鉴定目的蛋白邻近的蛋白或RNA...
TurboID邻近标记(Proximity labeling, PL)的原理是将TurboID生物素连接酶与诱饵蛋白融合,当诱饵蛋白与靶标蛋白发生互作时,TurboID生物素连接酶通过催化的共价修饰将邻近的蛋白标记上生物素,最后通过亲和素磁珠富集生物素标记的蛋白进行质谱鉴定,从而获得靶标蛋白信息。
Here, we used proximity labeling (PL) to identify the proteome proximal to N, which is an NLR that confers resistance to(TMV). Evaluation of different PL methods indicated that TurboID-based PL provides more efficient levels of biotinylation than BioID and BioID2 in plants. TurboID-based PL...
TurboID邻近标记(Proximity labeling, PL)的原理是将TurboID生物素连接酶与诱饵蛋白融合,当诱饵蛋白与靶标蛋白发生互作时,TurboID生物素连接酶通过催化的共价修饰将邻近的蛋白标记上生物素,最后通过亲和素磁珠富集生物素标记的蛋白进行质谱鉴定,从而获得靶标蛋白信息。
Enzyme-catalyzed proximity labeling has rapidly become a popular approach for studying the spatial localization and interactions of proteins in the cell. In this technique, a protein of interest is labeled with a promiscuous enzyme that adds a tag such as biotin to near-neighbor proteins, thereby ...
最近描述的基于 TurboID 的 PL 解决了 BioID 和 APEX 的许多限制。TurboID 允许在室温 (RT) 条件下只需 10 分钟即可快速贴贴蛋白质标签。尽管 TurboID 的效用已在动物模型中得到证明,但我们最近发现,与 BioID 相比,基于 TurboID 的 PL 在植物中性能更好,用于标记接近感兴趣的蛋白质的蛋白质。此处提供了一个...
文献阅读-1:Exploiting effector-host interactions using TurboID-based proximity labeling 1只小脑斧ya 总要坚持些什么吧1 人赞同了该文章 科学问题:Ustilago maydis效应子UmSee1是玉米感染期间叶片中肿瘤形成所必需的。UmSee1与玉米SGT1相互作用并阻断其在体内的磷酸化。然而,目前尚不清楚哪些宿主过程被UmSee1和Um...
A TurboID-based proximity labeling approach was deployed to gain mechanistic insights into the reprogramming of the HCC microenvironment. Results: We identified... Y Meng,F Ye,Nie P.Zhao Q.An L.Wang W.Qu S.Shen Z.Cao Z.Zhang X.Jiao S.Wu D.Zhou Z.Wei L. - 《Journal of Hepatology ...
TurboID邻近标记(Proximity labeling, PL)的原理是将TurboID生物素连接酶与诱饵蛋白融合,当诱饵蛋白与靶标蛋白发生互作时,TurboID生物素连接酶通过催化的共价修饰将邻近的蛋白标记上生物素,最后通过亲和素磁珠富集生物素标记的蛋白进行质谱鉴定,从而获得靶标蛋白信息。