Yanting S, Yuanyuan G, Jieyu G, et al. Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology.[J]. BMC genomics, 2023,24(1).
2023年,Yanting Su等人在期刊BMC Genomics上发表了(IF=4.4)题为“Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology”的文章。文章采用基于TurboID的邻近标记技术筛选并验证了与FOXO1相互作用的蛋白。TurboID作为一种生物素连接酶,具有比BioID或APEX更高的催化效率,可将所需的标记时...
Here, we used proximity labeling (PL) to identify the proteome proximal to N, which is an NLR that confers resistance to(TMV). Evaluation of different PL methods indicated that TurboID-based PL provides more efficient levels of biotinylation than BioID and BioID2 in plants. TurboID-based PL...
Using TurboID-based proximity labeling to analyze proximal proteins of bait proteins, we can study transient or weak protein interactions, which helps to understand the complex biological processes occurring inside cells. Here, we describe methods and related tips for TurboID-based proximal labeling in...
To determine whether the N NLR interacting proteins identified from TurboID-based proximity labeling function in theN-mediated resistance to TMV, we selected 10 proteins (Supplementary Table1) from the MS dataset for the subsequent genetic screening. For this, we selected proteins that spanned the ...
2019年7月19日,Nature Communications在线发表了中国农业大学张永亮课题组、加州大学戴维斯分校Savithramma P. Dinesh-Kumar课题组和爱荷华州立大学Justin W. Walley课题组合作题为“TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity”的研究论文。该研究首...
邻近标记(proximity labeling, PL)技术的基本原理是将一个具有连接酶活性的酶(目前植物中广泛应用的为TurboID)与目的蛋白融合, 在连接酶的催化作用下将小分子底物(如生物素(biotin))连接到与目的蛋白邻近的内源蛋白或RNA, 而后可通过富集被标记的蛋白或RNA分别进行质谱分析或RNA-Seq, 可鉴定目的蛋白邻近的蛋白或RNA...
Read this JoVE article on TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
TurboID邻近标记(Proximity labeling, PL)的原理是将TurboID生物素连接酶与诱饵蛋白融合,当诱饵蛋白与靶标蛋白发生互作时,TurboID生物素连接酶通过催化的共价修饰将邻近的蛋白标记上生物素,最后通过亲和素磁珠富集生物素标记的蛋白进行质谱鉴定,从而获得靶标蛋白信息。
TurboID邻近标记(Proximity labeling, PL)的原理是将TurboID生物素连接酶与诱饵蛋白融合,当诱饵蛋白与靶标蛋白发生互作时,TurboID生物素连接酶通过催化的共价修饰将邻近的蛋白标记上生物素,最后通过亲和素磁珠富集生物素标记的蛋白进行质谱鉴定,从而获得靶标蛋白信息。