Proximity-labelling technology has been widely used to identify partner proteins12,13,14. Since proximity labelling detects proteins that are very close together, it possibly obtains more precise information about interacting proteins15,16. Currently, several proximity-labelling-based techniques, such as ...
FOXO1Biotin labelingU251 cellshnRNPKProteinprotein interactions (PPIs) are the foundation of the life activities of cells. TurboID is a biotin ligase with higher catalytic efficiency than BioID or APEX that reduces the required labeling time from 18h to 10min. Since many proteins participate in ...
Filling the void: proximity-based labeling of proteins in living cells Trends Cell Biol, 26 (2016), pp. 804-817 10.1016/j.tcb.2016.09.004 27667171 View in ScopusGoogle Scholar 31 B.T. Lobingier, R. Hüttenhain, K. Eichel, K.B. Miller, A.Y. Ting, M. von Zastrow, N.J. Krogan ...
Complementary to traditional AP-MS methods, biotin-ligase-based proximity labeling technology is a powerful tool to investigate proximal protein interactions in living cells and organisms (Branon et al., 2018; Doerr, 2018; Kim et al., 2016; Larochelle et al., 2019; Roux et al., 2012; ...
The development of proximity labeling technology and its applications in mammals, plants, and microorganisms Jieyu Guo Shuang Guo Wu Liu Cell Communication and Signaling (2023) Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding Kohdai...
Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology Proteinprotein interactions (PPIs) are the foundation of the life activities of cells. TurboID is a biotin ligase with higher catalytic efficiency than Bio... Y Su,Y Guo,J Guo,... - 《Bmc Genomics》 被引量:...
Read this JoVE article on TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Norstat now offers an Ultrasonic Fork Sensor that is ideal for the detection of transparent labels or opaque label materials making it a suitable solution for packaging or labeling applications where the detection of the label compared to the carrier material is crucial. Ultrasonic sensing is one of...
the utilization of toxic H2O2during labeling, as well as high endogenous plant peroxidase activity, make it unsuitable for plants. The use of toxic H2O2is bypassed in the BioID-based labeling system, but longer incubation time with biotin (16–24 h) and higher incubation temperature (37 ...
Proximity-labeling-based proteomic approaches have been recently applied to plant research and have been shown to be invaluable in profiling functional components of various protein complexes10,11,12,13,14. By combining the power of BioID2-based proximity labeling (PL)15, label-free quantitative ma...