近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为"APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation"(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋白...
大家好,本周跟大家分享一篇发表在Angew上的文章,文章题目是“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”。本文通讯作者是来自北京大学药学院...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为“APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation”(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atox1细胞核内铜结合蛋...
Here, we present a new method based on semi﹑ermeabilized yeast cells. Combined with stable isotope labeling by amino acids in cell culture (SILAC), we demonstrate proteomic mapping of a membrane〆nclosed and a semi﹐pen compartment, the mitochondrial matrix and the nucleus. APEX2 PL revealed...
A genetically encoded peroxidase with improved sensitivity, APEX2, is reported for electron microscopy and proximity labeling at low expression levels. APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeli
Genetically targeted proximity labeling approaches for cell-type specificity and subcellular localization To filter out proteins that were enriched non-specifically by the beads, we defined the first filter based on comparisons between Cre-positive samples and Cre-negative control samples using a pairwise...
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is far mor...
Reminiscent of their evolutionary origin, mitochondria contain their own genome (mtDNA) compacted into the mitochondrial chromosome or nucleoid (mt-nucleoid). Many mitochondrial disorders are characterized by disruption of mt-nucleoids, either by direct mutation of genes involved in mtDNA organization or...
近日,药学院天然药物及仿生药物国家重点实验室王晶团队在学术期刊《Angewandte Chemie International Edition》发表了题为"APEX2-based Proximity Labeling of Atoxl Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation"(利用APEX2邻近标记技术鉴定铜伴侣蛋白Atoxl细胞核内铜结合蛋白...