The assay, referred to as proximity‐based labeling of membrane゛ssociated proteins (PLiMAP), is based on UV activation of a fluorescent lipid reporter, which in turn crosslinks with proteins bound to membranes and renders them fluorescent. 2020 Wiley Periodicals LLC. Basic Protocol 1: Synthesis...
We optimized TurboID-based proximity labeling inN. benthamianato enable identification of protein interactions partners (Supplementary Fig.3).N. benthamianaplants were infiltrated with agrobacterium containing the biotin ligase-fusion construct and at 36 hpi biotin was infiltrated and the plants were incubat...
Proximity-based labelling for proteomic mapping 来自 Springer 喜欢 0 阅读量: 1 作者: Z Jablonska 摘要: In this Tools of the Trade article, Jablonska describes the use of proximity-based labelling for the proteomic profiling of novel protein–protein interactions. DOI: 10.1038/s41569-024-01054...
Trinkle-Mulcahy, L. Recent advances in proximity-based labeling methods for interactome mapping.F1000Researchhttps://doi.org/10.12688/f1000research.16903.1(2019). Kim, D. I. et al. An improved smaller biotin ligase for BioID proximity labeling.Mol. Biol. Cell27, 1188–1196 (2016). CASPubMed...
文献阅读-1:Exploiting effector-host interactions using TurboID-based proximity labeling 1只小脑斧ya 总要坚持些什么吧1 人赞同了该文章 科学问题:Ustilago maydis效应子UmSee1是玉米感染期间叶片中肿瘤形成所必需的。UmSee1与玉米SGT1相互作用并阻断其在体内的磷酸化。然而,目前尚不清楚哪些宿主过程被UmSee1和Um...
Read this JoVE article on TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Filling the void: proximity-based labeling of proteins in living cells Trends Cell Biol, 26 (2016), pp. 804-817 10.1016/j.tcb.2016.09.004 27667171 View in ScopusGoogle Scholar 31 B.T. Lobingier, R. Hüttenhain, K. Eichel, K.B. Miller, A.Y. Ting, M. von Zastrow, N.J. Krogan ...
Filling the void: proximity-based labeling of proteins in living cells Trends Cell Biol., 26 (2016), pp. 804-817 Google Scholar Klemm et al., 2011 E.J. Klemm, E. Spooner, H.L. Ploegh Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticu...
Zhang, Y. et al. TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity.Nat. Commun.10, 3252 (2019). Larochelle, M., Bergeron, D., Arcand, B. & Bachand, F. Proximity-dependent biotinylation mediated by TurboID to identify protein-pr...
Proximity labeling-based proteomics (proximity proteomics) has emerged as a popular and versatile approach to illuminate the molecular interactions between viruses and their hosts. In this approach, a proximity labeling enzyme tag is fused to a bait protein and labels neighboring proteins with a ...