The effect of the mimics on permeability in vivo was assessed by the Miles assay.10 Mice were injected intradermally with 4 µg of the miR-23 mimics, LNA-23 or controls and 24 hours later assessed for response to VEGF. Mir-23a mimic significantly inhibited both the basal permeability and...
Mechanistically, enhancement of miR-23a by transfection with mimics in Aspc-1 cells significantly promoted cell proliferation and invasion, while miR-23a inhibitors transfection in SW1990 cells induced an inhibitory effect. Moreover, restoration of FOXP2 impaired the pro-proliferation and proinvasion ...
rat primary myocardial cells were transfected with miR-23a mimics after they were subjected to hypoxia-reoxygenation (H/R) injury. Transfection of miR-23a mimics stimulated mitophagy through the PINK1/Parkin pathway and downregulated the protein level of CX43. Treatment of miR-23a-transfected cells ...
Moreover, we observed that cell apoptosis was increased by miR-23a/b inhibitors and decreased by miR-23a/b mimics in gastric cancer cells and that the restoration of PDCD4 expression attenuated the anti-apoptotic effects of miR-23a/b in gastric cancer cells, indicating that PDCD4 is a ...
Transfection with miR-23a mimics were conducted when PASMCs were under normoxia and anoxia conditions. EdU staining was used to detect the proliferative activity of PASMCs. Cells were transfected with HIF-1α specific siRNA under anoxia condition. RT-qPCR was used to detect miR-23a expression in ...
miR-23a promotes the expression of IL-6 and IL-8 in human ARPE-19 cells. To elucidate the role of miR-23a in regulating the cytokine production of IL-6, IL-8 and MCP-1 in human RPE cells, cells were trans- fected with miR-23a mimics as well as inhibitors to affect the ...
The cells were then transfected with miR-23a mimics, inhibitors, or related NC (Fig. 1B). The transfection efficiency of mimic-NC and inhibitor-NC was determined to be above Discussion Follicular atresia is observed at each stage of the follicular development, and abnormalities in non-dominant ...
regulate luciferase activity; the down-regulation of circ_SEC23A effectively reversed the inhibition of H 2O 2 on miR-30a expression; H 2O 2 inhibited cardiomyocyte viability and promoted apoptosis, while circ_SEC23A siRNA and miR-30a mimics significantly blocked the above effects of H 2O 2....
(Shanghai, China). After inoculating ZR-75-1 cells into 96-well plates, luciferase reporter plasmids and either miR-NC or hsa-miR-23a-5p mimics were co-transfected into cells. Using the dual luciferase reporter gene assay kit (Promega, USA), relative luciferase activity was determined after ...