MiR-23a mimics repressed the induction of IL-1β and TNF-α levels and ex pression of p-p65. Results from TargetScan and luciferase assays showed that the 3-untranslated region (UTR) of PDE10A was directly targeted by miR-23a. The in vivo studies showed that miR-23a ag...
miR-23a promotes the expression of IL-6 and IL-8 in human ARPE-19 cells. To elucidate the role of miR-23a in regulating the cytokine production of IL-6, IL-8 and MCP-1 in human RPE cells, cells were trans- fected with miR-23a mimics as well as inhibitors to affect the ...
Moreover, we observed that cell apoptosis was increased by miR-23a/b inhibitors and decreased by miR-23a/b mimics in gastric cancer cells and that the restoration of PDCD4 expression attenuated the anti-apoptotic effects of miR-23a/b in gastric cancer cells, indicating that PDCD4 is a ...
The cells were then transfected with miR-23a mimics, inhibitors, or related NC (Fig. 1B). The transfection efficiency of mimic-NC and inhibitor-NC was determined to be above Discussion Follicular atresia is observed at each stage of the follicular development, and abnormalities in non-dominant ...
同样,普通A549 细胞系过表达miR-23a后其对放疗的敏感性亦显著降低;而抑制放疗抵抗A549细胞系miR-23a的表达后该细胞对放疗的敏感性增加。由于miR-23a表达增加可以增强癌细胞对放疗的抗性,从而导致放疗治疗的效果降低。在化疗相关影响因素的研究中,李杰等[27]在肺腺癌A549细胞中转染miR-23a mimics,研究miR-23a如何影响...
研究显示,内质网应激促进肝癌细胞释放外泌体,外泌体中miR-23a-3p上调巨噬细胞PD-L1表达,巨噬细胞PD-L1表达升高抑制CD8+ T细胞活性进而促进肝癌细胞逃避免疫监视[8]。 外泌体PD-L1在肿瘤免疫逃避中占有重要地位,肿瘤细胞可通过外泌体直接转运PD-L1,调控周围细胞如巨噬细胞功能促进肿瘤免疫逃避[9]。研究指出,胶质...
10 Mice were injected intradermally with 4 µg of the miR-23 mimics, LNA-23 or controls and 24 hours later assessed for response to VEGF. Mir-23a mimic significantly inhibited both the basal permeability and the VEGF-induced increase in vascular permeability (Figure 4h). MiR-23b mimic ...
3D, E). Analogously, the transfection of miR-1246 mimics into LX2 cells enhanced the migration and proliferation of CRC cell lines in subsequent tests (Supplementary Fig. 2A, B). Collectively, these results provide compelling evidence that CRC-secreted exosomal miR-1246 can activate HSCs, thereby...
见图4。过表达miR-338-5p后,与阴性对照相比,HCT116和SW620细胞的总凋亡率升高,差异具有统计学意义(miR-338-5p-mimics比阴性对照:HCT116为(11.43±0.67)%比(7.98±0.36)%,P < 0.01;SW620为(10.5±0.2)%比(7.93±0.5)%,P < 0.01)。提示miR-338-5p促进细胞凋亡与增殖结果一致。 图4 过表达miR-338-5p...