双荧光素酶报告结果显示：与转染mimic NC 和野生型KCNA1(KCNA1 WT)组相比，共转染6 nmol/L 或 20 nmol/L 的rno-miR-21-5p mimics 的KCNA1 WT 组的荧光素酶活性显著降低(P <0.001)；与6 nmol/L rno-miR-21-5p mimics 共转染组相比，较大剂量(20 nmol/L)的rno-miR-21-5p mimics 共转染组...
1.1 材料 人乳腺癌细胞株HCC1937购自中科院上海细胞库,通过Doc剂量阶梯递增方法建立稳转Doc耐药HCC1937/Doc细胞株,在中国医科大学肿瘤医院(辽宁省肿瘤医院)中心实验室培养传代。miR-21-5p mimics、miR-21-5p inhibitor、control mimics及control inhibitor购自英国Cohesion Biosciences公司,PDCD4多克隆抗体购自美国Sigma公司。
In addition, miR-21-5p inhibitors replicated the anti-fibrotic effects of pioglitazone, and miR-21-5p mimics inhibited these effects. In in vivo study, pioglitazone attenuated UUO-induced renal fibrosis and significantly decreased the expressions of pro-fibrotic proteins. Whereas, agomir of miR-21-...
Mimics-NC was the negative control for miR-21-5p-mimics and inhibitor-NC was the negative control for miR-21-5p-inhibitor. b, c Adhesion (Scale bar = 100 µm) and invasion (Scale bar = 100 µm) assays showed the effect of miR-21-5p on the HMrSV5 in vitro. d,...
and primers of mouse KRIT1 3′UTR were designed using Primer Premier 5.0. The KRIT1 3′UTR plasmids were co-transfected with mimics or inhibitor of miR-21-5p into the cells using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). After 48 h, luciferase activity was measured by the Du...
The augmentation of miR-21-5p expression, either through mimics or exosome-based delivery, has consistently demonstrated a reduction in infarct size and the preservation of cardiac function in AMI animal models [17]. A key underlying mechanism for these benefits is the ability of miR-21-5p to ...
NC组),EC109细胞转染miR-21-5pmimics与巨噬细胞共培养组(Exo-miR-21-5p组), M2型巨噬细胞组(M2macrophage组),M2型巨噬细胞加miR-21-5pinhibitor组 (M2+miR-21-5pinhibitor组),建立Transwell小室共培养模型,共培养24h后,发现 外泌体miR-21-5p可促进M0型巨噬细胞向M2型极化。
To verify their targeting relationship, the Dual-Luciferase report assays were performed after cotransfection for 48 h, and the results showed that the fluorescence activity of the SOX5-WT vector treated with miR-21-5p mimics was significantly lower than with NC. Meanwhile, the fluorescence of ...
We transfected osteoblasts separately with miR-21 mimics or inhibitors to explore the function of miR-21. EdU incorporation revealed that, at 48 hours after exosome (100 ng/µL) treatment, the proportion of proliferating osteoblasts had increased, and cell-viability analysis (CCK-8 test) also ...
biomolecules Article In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells Jung-Chien Chen 1,2,3,†, Yao-Yu Hsieh 1,4,5,†, Hsiang-Ling Lo 1,6, Albert Li 1,6,7 , Chia-Jung Chou 1,6,8 and Pei-Ming ...