miR-140-5p mimics could downregulate the wnt1 mRNA and protein levels in MCF-7 and MDA-MB-231 cells. Furthermore, miR-140 mimics could enhance the sensitivity of BCSCs to doxorubicin (Dox) through the Wnt1/ABCB1 pathway both in vitro and vivo. Our findings have presented a novel miRNA...
结果 miRNA-140-5p在细胞MDA-MB-231缺氧状态中的表达降低(P<0.05),转染miRNA-140-5p mimics表达升高.miRNA-140-5p mimics能增强细胞在低氧环境中的增殖,迁移,侵袭能力;miRNA-140-5p mimics也能增强NOX4在MDA-MB-231中的表达影响.结论 miRNA-140-5p在乳腺癌细胞MDA-MB-231缺氧状态中的表达减低,在转染Mimic...
The recombinant vector was transfected into 293 T cells using the pofectamine 3000 reagent (L3000015, Thermo, USA), along with mimics NC and miR-140-5p mimics. Subsequently, luciferase activity was measured using the Nano-Glo dual-luciferase reporter assay. Chromatin immunoprecipitation (ChIP)-...
The next day, the cells were transfected with 50 nM of miR-140-5p mimics or negative control mimics (Thermo Fischer) using Dharmafect1 (Thermo Scientific) following the manufacturer’s protocol. Simultaneously, the cells were transfected with luciferase constructs at a concentration of 100 ng per...
Furthermore, miR-140-5p mimics did not reduce the sensitivity of PIN1mut plasmid to DOX in HUH7 and SNU449 cells. The present study demonstrated that miR-140-5p could enhance DOX sensitivity in hepatocellular carcinoma cells by targeting PIN1....
related signaling pathways, suggesting a mechanism by which miR-140-5p may influence SARS-CoV-2 entry.Experimental validation using miR-140-5p mimics demonstrated a significant reduction in viral entry across multiple SARS-CoV-2 variants, confirming the inhibitory role of miR-140-5p on viral ...
19.The expression of miR-140-5p was up-regulated in MG-63 and U20S cells which were transfected with miR-140-5p mimics, then the ability of invasion and migration of MG-63 and U20S cells was decreased. Conclusion The expression of miR-140-5p is down-regulated...
用NF-κB信号通路激活剂处理LPS条件下转染miR-140-5p mimics的人牙髓细胞,ELISA法检测培养液上清中IL-6,IL-1β,TNF-α含量,Western Blot检测细胞中p-IκBα,IκBα,NF-κBp65蛋白表达.结果:与对照组比较,LPS组细胞中miR-140-5p水平下降,细胞培养液上清中IL-6,IL-1β,TNF-α含量升高,细胞中p-IκBα,...
The role of miR-140-5p in CBSCs was evaluated by transfection of mimics and inhibitor. RNA sequencing and online search of miRNA databases (TargetScan, miRDB and miRanda) were used in prediction of miR-140-5p targets. A luciferase reporter assay was applied to identify the target gene of ...
Moreover, transfection of miR-140-5p mimics significantly suppressed the expression of Smad3 and TGF-β3 in MCCs, while miR-140-5p inhibitors acted in a converse manner. As the luciferase reporter of Smad3 mRNA observed active interaction with miR-140-5p, Smad3 was identified as a direct ...