2024年5月,钟一声主任医师在《Journal of Translational Medicine》【IF6.1,Q1】杂志发表题名为“MiR-146a reduces fibrosis after glaucoma filtration surgery in rats.”——MiR-146a降低大鼠青光眼滤过术后纤维化的研究论文。 瑞金医院眼科...
The NF-魏B signaling pathway was inhibited by miR-146a mimic. miR-146a treatment attenuated the impact caused by TBI to mouse brain and improve the long-term neurological function. In conclusion, miR-146a mimics ameliorate TBI-related injuries via JNK and NF-魏B signaling pathway....
correlated with tumor size and clinical stages. The patients with high miR-146a expression levels had significantly longer total survival time than those with low expression of miR-146a. In SW260 cell cultures, transfection with miR-146a mimics significantly inhibited cell growth (P<0.05) and incre...
(4)pdasp-cy3oligo的复合物制备:cy3oligo的加入量和mirna-146amimics的摩尔量一样。 (5)体外转染复合物的操作方法: 转染6孔板:每孔加入2ml的无酚红的dmem,再加入400μl的复合物溶液(6孔板为400μl,96孔板为25μl,其他孔板按比例计算),轻轻米字型震摇培养板,放入培养箱中孵育4小时后,弃去培养基,用pbs缓冲...
方法:获取雄性C57BL/6小鼠脂肪间充质干细胞,采用倒置显微镜观察细胞形态,流式细胞学方法检测第3代细胞CD29、CD44、CD45的表达,然后用miR-NC、miR-146a mimics和miR-146a inhibitor转染脂肪间充质干细胞并进行成骨诱导分化,成骨诱导第6天,采用碱性磷酸酶染色观察钙化程度,RT-PCR和Western blot检测成骨表型标志物的...
与NC组和NC-mimics组比较,miR-146a-3p mimics组星形胶质细胞凋亡率增加(P < 0.01),增殖能力下降(P < 0.01),迁移数量减少(P < 0.01)。结果表明,脊髓损伤后脊髓组织中miR-146a-3p表达量下降,胰岛素样生长因子1表达量上升;在星形...
Background:The prevalence of allergic asthma has increased dramatically. Previous studies have found that the microRNA 146a (miR-146a) expression in asthma inhibits cell proliferation and promotes apoptosis of bronchial smooth muscle cells. We aimed to investigate the effect of miR-146a mimics on ova...
如附表显示,miR一146a mimics能够显著抑制细胞内Tc含量、CE含量和CE/TC比值,而miR-146ainhibitor则显著增加细胞内Tc含量、CE含量和CE/TC比值,Mimic—neg和Inhitior.neg无显著影响,提示miR.146a参与调控巨噬细胞内的胆固醇平衡.对巨噬细胞的泡沫化具有重要影响。附表miR一146a对THP—l巨噬细胞源性泡沫细胞胆固醇的...
目的:探讨miR-146a对 小鼠单核-巨噬细胞株RAW264.7以及小鼠原代腹腔巨噬细胞Thl/Th2类细胞因子表达的影响.方法:体外培养的小鼠单核-巨噬细胞系 RAW264.7细胞和腹腔新鲜分离的巨噬细胞分别瞬时转染miR-146a mimics,阴性对照mimics(NC mimics),抑制性miR-146a(miR-146a inhibitor)和抑制性miR-146a阴性对照(NC in-...
ｍｉＲ １４６ａ对高糖诱导的人视网膜微血管内皮细胞增殖和新生血管生成的影响及其机制△ 何 静 谢 平 欧阳君 肖婷婷 徐琰瑛 袁 晴 欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁氉氉 氉 氉 作者简介：何静（ＯＲＣＩＤ：００００...