miR-137 mimics had greatly suppressed ability to invade matrigel in LN229 cell,exhibiting about 44. 4 卤 6. 8,42. 2 卤 6. 1,16. 9卤 2. 1 respectively in blank group,negative control group and mimics treated group cells. Moreover,Tcf-4 protein expression...
For example, delivery of miR- 34 and let-7 mimics inhibited tumor development in mouse models of lung and prostate cancers.119–121 Some other specific miRNAs currently in development as clinical candidates include miR-155, miR-21 and miR-33.122 Although miR-137 also presents as a promising ...
目的探讨mir-137对非小细胞肺癌a549细胞侵袭迁移的影响及其可能机制.方法将a549细胞随机分为空白对照组(不进行任何处理),mir-137模拟物组(转染mir-137 mimics)与阴性对照组(转染无关序列).按照脂质体说明方法进行转染,实时荧光定量pcr方法验证转染效率.转染后,采用transwell实验与划痕实验检测a549细胞侵袭与迁移能力变化...
MiR-137 is a classic 23-nt microRNA located within a long non-coding host gene,MIR137HG. This transcriptional unit on chromosome 1 is over 62 kb long and is predicted to produce four splice variants ranging from over 2.5 kb to below 1 kb. There is also a 15-bp variable tandem...
the result was quantified by comparing with the internal control U6.c–gDetection of viability of BC cells transfected with miR-137 mimics (5 nM) or negative control (NC) RNA and cultured with 0, 0.5, 1.0, 1.5, and 2.0 µg/ml DOX for 48 h.h–lDetection of viability of BC...
Direct transfection of miR-137 mimics is more effective than DNA demethylation of miR-137 promoter to augment anti-tumor mechanisms of delphinidin in human glioblastoma U87MG and LN18 cells Gene, 573 (2015), pp. 141-152 View PDFView articleView in ScopusGoogle Scholar 54 D. Li, W. Shan,...
(ECM)积聚的作用.以人肾小球系膜细胞(HRMC)为对象,验证TP对ECM蛋白的作用,通过miRNA芯片筛选TP干预下差异明显的miRNA.通过预测软件,筛选所选定miRNA的靶基因,以双荧光素酶验证二者作用.分别向HRMC转染miR-137mimics和inhibitor,观察对ECM蛋白的影响,并通过抑制剂和小干扰RNA(siRNA)探讨作用机制:观察miR-137对TP干预...
Our observation that upregulating tumor suppressive miR-137 and miR-34a via miRNA mimics restored tumor suppressor activity, with successful inhibition of OC cell viability and invasiveness, provided a rationale to investigate “miRNA replacement therapy for miR-137 and miR-34a”. However, there are ...
cell viability and the induction of cell apoptosis, but had no significant toxicity to NOEC cells (Fig. 5), indicating the feasibility of inhibiting the growth of OC cells via the therapeutic delivery of miR-137 or miR-34a mimics, with- out causing significant toxicity to normal ovarian ...
To assess the function of candidate microRNAs in high-grade astrocytomas, we transfected miR mimics to cultured-mouse neural stem cells, -mouse oligodendroglioma-derived stem cells, -human glioblastoma multiforme-derived stem cells and -glioblastoma multiforme cell lines. Cellular differentiation was ...