1.2 转染试验 对数生长期HCC1937及HCC1937/Doc细胞消化为单细胞悬液,以20×104/孔细胞接种于24孔板,依据Lipo2000说明书对达到70%融合度的细胞株转染(miR-21-5p mimics、miR-21-5p inhibitor、control mimics及control inhibitor),转染过程完成后继续培养,48 h后qRT-PCR进行转染效率检测,准备进行下一步实验。 1.3 ...
与sham 组相比，TN 组TG 中Kv1.1 mRNA 和蛋白质表达均下调(均P <0.05)，miR-21-5p 的表达上调 (P <0.05)。双荧光素酶报告结果显示：与转染mimic NC 和野生型KCNA1(KCNA1 WT)组相比，共转染6 nmol/L 或 20 nmol/L 的rno-miR-21-5p mimics 的KCNA1 WT 组的荧光素酶活性显著降低(P <0.001...
To further investigate whether miR-21-5p could mediate MMT of HMrSV5 cells, HMrSV5 cells were transfected diferent lentvirus contructs, including miR-21-5p mimics, negative control for mimics, miR-21-5p inhibitors or negative control for inhibitors. As shown in Fig. 4a, miR-21-5p levels ...
MiR-21-5p mimics could promote cell proliferation, migration, and invasion of ASPC-1, as well as decrease the expression of B cell translocation gene 2. Curcumin treatment inhibited cell proliferation, migration and invasion of ASPC-1, as well as increased the e...
and primers of mouse KRIT1 3′UTR were designed using Primer Premier 5.0. The KRIT1 3′UTR plasmids were co-transfected with mimics or inhibitor of miR-21-5p into the cells using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). After 48 h, luciferase activity was measured by the Du...
3.外泌体miR-21-5p促进巨噬细胞M2型极化 设置四个实验组:EC109细胞转染miR-NCmimics与巨噬细胞共培养组(Exo-miR- NC组),EC109细胞转染miR-21-5pmimics与巨噬细胞共培养组(Exo-miR-21-5p组), M2型巨噬细胞组(M2macrophage组),M2型巨噬细胞加miR-21-5pinhibitor组 (M2+miR-21-5pinhibitor组),建立Trans...
Neither GH nor IGF-1 increased miR-21-5p expression in hFOB1.19 cells when compared with the miR-21-5p mimics (Fig S2). These results indicate that miR-21 might have been the effector molecule contained in the GHPA exosomes and that miR-21 transportation from GH3 cells to osteoblasts may...
本研究结果显示miR-146a-5p在FTC组织中的表达水平低于癌旁组织,提示miR-146a-5p在FTC的发生中有一定的参与作用。转染试验结果显示与miR-NC组相比,miR-146a-5p mimics组的miR-146a-5p相对表达量较高。且细胞活力会随着培养时间的延...
宫颈癌SiHa细胞分别共转染miR-3619-5pmimics与阴性对照载体、miR-3619-5pmimics与MIEN1过表达载体,然后用含有100 μg/mL的马钱苷处理24 h,记为马钱苷+miR-3619-5p+Vector组和马钱苷+miR-3619-5p+MIEN1组。按“2.4”项下方法检测细胞...
ZNF521促进结肠癌的进展且受miR-211-5p靶向调控