在双荧光素酶报告实验中,2种psiCHECK-2和miRNA-mimics/miRNA-NC被共同转染。如图4D所示,与miR-29c-3p-NC组的效果相比,miR-29c-3p过表达明显抑制了共转染的psiCHECK-2-WT组的相对荧光素酶活性。然而,在psiCHECK-2-MUT组中无显著差...
LPS-induced ALI mouse models were treated with BALF-exo loaded with miR-223-3p mimics. miR-223-3p was lowly expressed in BALF-exo from LPS-treated mice. BALF-exo loaded with miR-223-3p mimics increased viability and autophagyand decreased apoptosis and inflammation in NR8383 cell models. ...
After cultured in a incubator chamber at 37uC and 5% CO2, cells were transfected with miR-223-3p mimics, miR- 223-3p antisense oligonucleotides or miR-NC. MTT was diluted and 50 ul 13 MTT was dropped in to each plate after the transfected cells cultured 48 hours. The cells were ge...
转染miR-223-3p mimics的T细胞中,IL-17的产生,Th17细胞比例及致病性Th17细胞相关基因(IL-17,RORγt,IL-22,IL-1R1,IL-23R和GM-CSF)的表达均显著增加,而转染miR-223-3p inhibitor的T细胞中,IL-17的分泌,Th17细胞比例及致病性Th17相关基因的表达均明显减少.过继转移miR-223-3p inhibitor转染的Th17细胞的小鼠EAU...
Intrauterine adhesion (IUA) is the clinical manifestation of endometrial fibrosis. The dysregulation of microRNAs (miRNAs) has been confirmed to implicate in a diversity of human diseases, including IUA. Nevertheless, the specific function of miR-223-3p
The miR-223-3p over-expression group was transfected with miR-223-3p mimics. Proliferation rates of cells from each group were detected by the MTT and the plate colony formation assays. Apoptosis rates of cells in each group were detected by flow cytometry. Expression levels of the ECT2 ...
目的探讨miR-223-3p抑制人肝星状细胞LX-2增殖,迁移的作用及分子机制.方法以TGF-β1诱导活化的LX-2细胞为研究对象,分为空白对照组,miR-223-3p mimics组,NC mimics组,分别采用空白溶剂,miR-223-3p mimics,模拟物对照(NC mimics)转染干预24,48 h.采用CCK-8法检测miR-223-3p mimics对LX-2细胞增殖活性的影响...
PFN2 upregulation reversed the effect of miR-223-3p mimics. In animal models, knockdown of DUXAP8 inhibited tumor growth and enhanced radiosensitivity.DUXAP8/miR-223-3p/PFN2 axis modulated radiosensitivity in lung cancer.Pang, ChongTianjin’s Clinical Research Center for Cancer, Key Laboratory ...
结果miR-223-3p在低氧诱导的DPSCs细胞中表达增多(P<0.05).MTT结果表明miR-223-3p mimics对DPSCs细胞的增殖有促进作用(P<0.05).且miR-223-3p通过靶向VHL 3′-UTR调控VHL的表达水平.qRT-PCR和Western blot法检测miR-223-3p成功转染,显著促进HIF-1α,Runx2,BMP2基因的表达(P<0.05).结论miR-223-3p通过抑制VHL...
CircBNC2 can sponge miR-223-3p, andLARP4is the target of miR-223-3p. In-vitro complement experiments showed that overexpression of circBNC2 significantly decreased the malignant behavior of EOC, while co-transfection of miR-223-3p mimics partially upregulated this change. In addition,LARP4...