3. miR-34a对肝细胞系(BRL-3A)生物学功能及靶基因的影响 用miR-34a的mimics转染大鼠肝细胞BRL-3A后,用MTT法来检测miR-34a对大鼠肝脏细胞增殖调控的影响,在4天时可观察到miR-34a对BRL-3A细胞的增殖抑制作用,6天时抑制作用达到最高水平.实验结果证明了miR-34a可以抑制大鼠正常肝细胞增殖.随后,用miR-34a的...
miR-34a对人牙周膜干细胞成骨分化的促进作用及其机制 董霞1,王训霞1,杨芳2 （1.山东省青岛市海慈医疗集团口腔科，山东青岛266000；2.山东省青岛市立医院口腔医学中心，山东青岛266000）［摘要］目的 目的：探讨微小RNA-34a（miR-34a）对人牙周膜干细胞（PDLSCs）成骨分化的影响，并阐明其作用机制。方法 方法：...
方法乳腺癌细胞MCF-7转染miR-34a mimics及miR-34a NC,RT-PCR法检测细胞中miR-34a表达,MTT,克隆形成实验,Hoechst染色及流式细胞术分别检测miR-34a mimics对乳腺癌MCF-7细胞活力、克隆能力、凋亡情况及细胞周期的影响;通过双荧光素酶报告基因实验,western blot及RT-PCR检测Notch1是否是miR-34a的下游靶基因。结果与...
mimics上调miR一34a的表达并检测其细胞活力;在正常细胞HEK293中转染miR一34ainhibitors下调miR一34a的表达水平并 检测其细胞凋亡率。结果 照前辐射敏感性高的细胞miR一34a表达水平高,辐射敏感性低的细胞miR一34a表达水平低;照后 辐射敏感性高的细胞miR一34a表达上调幅度远高于辐射敏感性低的细胞。上调miR一34a表达水平...
We36,37and others38have reported that overexpression of transforming growth factor (TGF)-β1 in the developing lung mimics the BPD pulmonary phenotype. To determine whether the effects of miR-34a are limited to the hyperoxia-induced BPD model or could be dependent on other injury mediators, we...
Di Martino MT, Leone E, Amodio N, Foresta U, Lionetti M, Pitari MRet al. Synthetic miR-34a mimics as a novel therapeutic agent for multiple myeloma:in vitroandin vivoevidence.Clin Cancer Res2012;18: 6260–6270. CASPubMedPubMed CentralGoogle Scholar ...
et al. Synthetic miR-34a mimics as a novel therapeutic agent for multiple myeloma: in vitro and in vivo evidence. Clin. Cancer Res. 18, 6260–6270 (2012). PubMed PubMed Central Google Scholar Zhao, J., Kelnar, K. & Bader, A. G. In-depth analysis shows synergy between erlotinib ...
在 神经母细胞瘤细胞中瞬时转染miR-34amimics,利用平板克隆形成实验、MTT、侵 袭实验和体外划痕实验检测miR-34a对细胞生长、侵袭和迁移的影响。最后利用 Western印迹检测miR一34a对生长以及侵袭迁移相关蛋白表达水平的影响。 结果与原发性神经母细胞瘤组织相比,miR-34a在转移性神经母细胞瘤组织中表 达降低。与对照细胞...
and MIR34a levels in serum have been proposed as diagnostic biomarker for PDAC30,32,33,34. Additionally,Mir34ahas a great therapeutic potential35, and it has already been tested in pre-clinical studies, whereMir34amimics (in combination with PLK1 siRNA) was delivered using an amphiphilic nano-...
Pat there was a base compleme/Pp between miR-34a-3p and the3'UTR of DDX02.The results of Pc dxal luciferase expeUpeut showed Pat,compareO with miR-NC,the exopsceuce of miR-34a-3p- mimics co-transformed with Pc wilU-pyc3’UTR repion(WT-DDX92)was reXxeed,a—compareO with the ...