侵袭Smad2TGF-β目的 探讨miR-34a-3p对胰腺癌细胞的作用及可能机制.方法 RT-qPCR方法检测miR-34a-3p在人胰腺导管腺癌细胞SW1990和胰腺导管上皮细胞HPDE中的表达情况;转染miR-34a-3pmimics后,采用CCK-8法检测细胞增殖;Transwell小室实验检测细胞侵袭情况;Western blot检测细胞上皮-间质转化情况;进一步用生物信息学预测...
miR-34a-3p,DDX07a—Pc survival rate of colorectal cancer pakeuts.Mu/ivabate Cox repression was used to avalysio the ymgnos/c factors of colorectal caucer.Result::The online ppOipWb results of Targetscan wedsite showed Pat there was a base compleme/Pp between miR-34a-3p and the3'UTR of...
Upon binding of miR-34a-3p to theSMAD43’UTR, the luciferase activity was reduced to 75% (SEM ± 1.76, P < 0.001), and upon binding to theFRAT13’UTR the luciferase activity was reduced to 79% (SEM ± 1.72, P < 0.001). After disruption of the potential binding sites the response ...
目的 探讨miR-34a-3p通过靶基因YAP1调控黑色素瘤A375细胞的增殖和凋亡.方法 通过荧光定量PCR检测miR-34a-3p在黑色素瘤和正常皮肤组织中的表达,并通过双荧光素酶报告基因检测miR-34a-3p的靶基因,黑色素瘤A375细胞转入miR-34a-3p模拟剂(miR-34a-3p mimics组),转入对照物miR-NC(对照组miR-NC),miR-34a-3p mim...
LncRNA MEG3 aggravates acute pulmonary embolism-induced pulmonary arterial hypertension by regulating miR-34a-3p/DUSP1 axis LncRNA MEG3通过调节miR-34a-3p/DUSP1轴加重急性肺栓塞诱导的肺动脉高压 相关领域 肺栓塞 心脏病学 肺动脉高压 医学 内科学 ...
结论miR一34a分别通过降低CD44、CDK4、CCNDl和CCNE2的表达水平,而抑制神经母细胞瘤细胞的生长、侵袭和迁移,发挥着肿瘤抑癌基因的作用,提示miR-34a可能作为神经母细胞瘤分子治疗的靶点。关键词:miR一34a;神经母细胞瘤;侵袭博士研究生陈鑫(儿科学)指导教师董蓓教授 AbstraetPartITheinhibitionofmiR-338-3poncell...
目的 探讨妊娠期高血压(GH)患者血清miR-34a-5p,miR-488-3p表达水平及其与疾病严重程度,不良妊娠结局的关系.方法 纳入82例GH患者(GH组)及82例正常妊娠女性,并根据疾病严重程度将GH组患者分为单纯GH组37例,轻度子痫前期组(轻度PE组)28例,重度子痫前期组(重度PE组)17例,又根据妊娠结局将GH组患者分为妊娠结局良好...
摘要: 目的 探讨宫颈癌患者血清miR-329-3p和miR-34a-5p水平检测及临床意义.方法 选取201 1年3月-2014年8月义乌市中心医院宫颈癌患者154例为观察组,分别于手术前以及术后3～4周采集全血,另选健康体检者123例为对照组,体检时采血,测定其血清miR-329-3p和miR-34a-5p及癌胚抗原(CE... 查看全部>> ...
目的探讨宫颈癌患者血清miR-329-3p和miR-34a-5p水平检测及临床意义.方法选取2011年3月-2014年8月义乌市中心医院宫颈癌患者154例为观察组,分别于手术前以及术后3~4周采集全血,另选健康体检者123例为对照组,体检时采血,测定其血清miR-329-3p和miR-34a-5p及癌胚抗原(CEA)表达水平.结果术前观察组miR-329-3p,miR...
However, the effect of miR-34a/c-5p and miR-29b-3p on Snail and the intestinal barrier function has not been well studied. Mesenchymal stem cells (MSCs) play an important role in improving intestinal barrier dysfunction, but the specific mechanism is not clear. MSC-derived exosomes are known...