侵袭Smad2TGF-β目的 探讨miR-34a-3p对胰腺癌细胞的作用及可能机制.方法 RT-qPCR方法检测miR-34a-3p在人胰腺导管腺癌细胞SW1990和胰腺导管上皮细胞HPDE中的表达情况;转染miR-34a-3pmimics后,采用CCK-8法检测细胞增殖;Transwell小室实验检测细胞侵袭情况;Western blot检测细胞上皮-间质转化情况;进一步用生物信息学预测...
aca-miR-34a-3p qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:aca-miR-34a-3p 原价:￥4030 现价:￥3098.38 节省:￥932 规格:1 kits 说明:aca-miR-34a-3p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方...
摘要 目的探讨高危型急性肺血栓栓塞症(APTE)患者血清微小RNA(miR)-34a-3p和miR-106b-5p的表达和意义。方法选取该院2022年2月至2023年2月收治的85例高危型APTE患者作为研究组;选择同期在该院...展开更多 Objective To investigate the expression and significance of serum microRNA(miR)-34a-3p and miR-106...
结果观察组血清hsa-miR-34a-3p,hsa-miR-206水平高于对照组(P<0.05);随访至妊娠结束,观察组子代心肌损害的发生率为20.97%(52/248),对照组子代心肌损害的发生率为1.69%(4/236);发生组血清hsa-miR-34a-3p,hsa-miR-206水平高于未发生组(P<0.05);多因素Logistic回归分析显示血清hsa-miR-34a-3p,hsa-miR-206,...
In contrary to the common belief that only one strand of the pre-miRNA is active (usually the 5p one that is the more abundant) while the second one (miRNA*) is discarded, functional 5p and 3p have been observed for many miRNAs. Among those miRNAs is miR-34a which is a target gene ...
结论抑制miR一92的表达导致神经母细胞瘤细胞的增殖降低,凋亡增加,从而可能进一步影响神经母细胞瘤的生长,miR-92可能具有作为临床上神经母细胞瘤治疗分子标记物的潜在功能,为以后更有意义的分子治疗奠定基础。博士研究生陈鑫(儿科学)指导教师董蓓教授关键词:miR-92;神经母细胞瘤;增殖;PTEN 摘要第三部分miR一34a对神经...
第一部分 miR-338-3p对神经母细胞瘤的增殖和侵袭的抑制作用的研究 目的:探讨microRNA-338-3p(miR-338-3p)对神经母细胞瘤细胞系SK-N-SH和SK-N-MC增殖,侵袭和迁移能力的影响,miR靶基因的确定及靶基因功能的研究. 方法: miRNA芯片结果发现miR在神经母细胞瘤中低表达,利用实时定量PCR验证其结果.利用脂质体Lipofec...
Although miR-34a-5p and miR-545-3p might be promising early biomarker candidates for AD, our study highlights possible sources of variability in miRNA analysis across hospitals, which currently prevents their use as reliable clinical tools. 展开 关键词: miRNA Alzheimer’s disease Plasma biomarker ...
cells.miR-338-3p and miR-34a-5p act as antagonists in the phycocyanin-mediated process of T2DM amelioration.PTPRM and NR3C2 are direct targets of miR-34a-5p and miR-338-3p, respectively.miR-34a-5p/Ptprm and miR-338-3p/Nr3c2 axis are involved in phycocyanin-mediated glucose regulating ...
摘要: 目的 探讨宫颈癌患者血清miR-329-3p和miR-34a-5p水平检测及临床意义.方法 选取201 1年3月-2014年8月义乌市中心医院宫颈癌患者154例为观察组,分别于手术前以及术后3～4周采集全血,另选健康体检者123例为对照组,体检时采血,测定其血清miR-329-3p和miR-34a-5p及癌胚抗原(CE... 查看全部>> ...